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The Biochemical Oxygen Demand (BOD) of a sample is the amount of dissolved oxygen required by aerobic microorganisms to breakdown organic matter in the sample.
The test is simple:
Measure the Dissolved Oxygen content of the sample (a)
Place in a sealed darkened bottle
Incubate for 5 days at 20'C
Measure the Dissolved Oxygen content of the incubated sample (b)
Generally you're using this test to analyse contaminated water, so you'll want to dilute it first - it may be that aren't enough aerobic microorganisms present to give you a measurable result, in which case you'll need to inoculate the sample with a suitable culture.
The BOD of the sample = a - b
BOD is expressed in mg O2/L of sample.
Extra Credit
Generally you're using this test to analyse contaminated water, so you'll want to dilute it first - it may be that aren't enough aerobic microorganisms present to give you a measurable result, in which case you'll need to inoculate the sample with a suitable culture.
The BOD of a diluted innoculated sample = ((a - b) - BOD of inoculated culture) * dilution factor
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