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A compound has a very high Rf value in a polar solvent what can you say about its molecular structure?
Rf Value - It tells you the affinity that the compound has to the solvent...High Rf = little or no polar groups and low Rf = more polar groups. Question: In TLC (thin-layer chromatography) when the compound has a high Rf the solvent is: a. less polar b. Is more polar Answer: a. less polar
How do you check the volatility of any unknown compound?
the volatility of any unknown compound can be known by comparison method between the compound whose volatility is known by the use of paper chromatography. since the high volatile compound has less or no adsorptions to the stationary phase of the parer (the compound is less polar or non-polar) it will move high with the solvent, thus, having high Rf value. The polar compound or less volatile compound will have less Rf value. The conclusion therefore, can be made from the differences in their Rf values
How does saturation effect RF value?
In general, as saturation increases, the RF value decreases. This is because an increase in saturation tends to increase retention of the compound on the stationary phase, resulting in a lower RF value. Conversely, lower saturation levels may allow the compound to move more freely, leading to a higher RF value.
If a chromatogram is run for a longer period of timem will the Rf values increase for each pigment?
No, the Rf values will not increase for each pigment with a longer chromatography run time. The Rf value is a constant characteristic of a compound in a particular solvent system and is not affected by the duration of the chromatography run.
Why tryptophan has higher rf value?
Tryptophan has a higher Rf value in chromatography due to its relatively non-polar structure compared to other amino acids. The Rf value, or retention factor, is influenced by the compound's solubility in the mobile phase and its interaction with the stationary phase; tryptophan's hydrophobic side chain allows it to travel further in non-polar solvents. Additionally, its larger size and structure may contribute to its mobility, leading to a higher Rf value compared to more polar compounds.
What is the Rf value of decane?
The Rf value of decane will depend on the specific conditions of the chromatography experiment (type of solvent, type of stationary phase, etc). In general, decane is non-polar and tends to have a higher Rf value in non-polar solvents compared to polar solvents. Typically, the Rf value of decane in non-polar solvents is close to 1.
What solvent system would give the highest Rf value for acetophenone?
A non-polar solvent system, such as hexanes and ethyl acetate, would provide the highest Rf value for acetophenone since it is a non-polar compound. The non-polar solvent system will have less affinity for the polar silica gel in the TLC plate, allowing the acetophenone to move further up the plate during the chromatography process.
What is the rf value for lycopene?
The Rf value of Lycopene is between 0.4 and 0.6. The best mobile phase for Lycopene is a mixture of a polar and non-polar solvent.
What does it mean if the Rf value is 1?
In chromatography, the Rf value (retention factor) is a measure of the distance a compound travels relative to the distance the solvent front travels on a chromatography plate. An Rf value of 1 means that the compound traveled the same distance as the solvent front, indicating that the compound has no affinity for the stationary phase and is completely soluble in the mobile phase. This could suggest that the compound is non-polar or that the conditions of the chromatography were not suitable for proper separation.
Will acetaminophen or ibuprofen have a higher Rf if you carry out a TLC separation of on a silica gel using a non-polar developing solvent?
Acetaminophen will have Rf value of about 0.4 and ibuprofen will have Rf value of about 0.6 if the non polar solvent used is ethanol.
What is Rf value of o-nitroaniline?
The Rf value will vary some depending on the exact conditions. Using a silica plate and a moderately polar solvent (dichloromethane or a mixture of toluene/ethyl acetate) you will get an Rf of 0.4-0.5.
You run a TLC plate spotted with three compounds naphthalene o-toulic acid and fluorenol. Predict the relative Rf values?
You'd have to know which mobile phase is used before you can make a guess at the Rf values. It might be quite hard to find a decent mobile phase to have all those compounts running, naphtalene and fluorenol are bulky and have low affinity for the polar silica, where as o-toluic acid will stick to the bottom of the plate due the COOH group.
