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The different substances present in the ink have different Rf values (Rf value is the ratio of the distance the dissolved material traveled to distance the solvent traveled) the different substances each have a unique or nearly unique Rf value. This occurs because each material's chemical structure has a different solubility in the solvent,used to develop the chromatography plate,. This means that some compounds easily dissolve and are carried by the solvent while others are more attracted to the chromatgraphy plate and don't budge as easily.

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a chem major

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Q: How different colors are discovers what colors are in each ink using chromatography?
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