How do bacteria clone?

Answer 1

bacteria regenerates by cloning, their behaviour will be same and will become smaller, grow again, and again clones, it happens by... in a microscope it will look like a bactiria is been cut into two and will look smaller.

Answer 2

1. Sequence the DNA containing the eukaryotic gene and map it with known restriction sites.

2. Find a bacterial plasmid (also known as vector) with the corresponding restriction sites in it's MCS (multiple cloning site).

3. Design primers 5' and 3' to your eukaryotic gene that lie to OUTSIDE of your restriction sites.

4. Run a PCR reaction using your eukaryotic DNA as the template with your primers, buffer, dNTPs and your pet DNA polymerase.

5. Tailor you PCR product with the restriction enzymes, and cut your vector with the identical restriction enzymes.

6. Clone the tailored PCR product with the eukaryotic gene into the vector using DNA Ligase.

7. Transform bacterial competent cells with your ligation mixture, and select on plates containing the selection media for transformants.

Answer 3

bacteria clone by a asexual way called binary fission