How do colonies on the surface of a pour plate differ from those suspended in the agar?
How do colonies on the surface of a pour plate differ from those suspended in the agar?
Because of their ability to grow on specific environment & the amount of oxygen needed...(e.g. anaerobes, aerotolerants will grow in agar).
The surface colonies on a pour plate larger than those within the medium especially aerobic bacteria within the medium would be a restriction of growth. The restriction of growth would be due to the lack of oxygen.
The pour plate method often results in colonies developing both down throughout the agar and on the surface. This is because the pour plate involves mixing the bacteria with the agar before pouring it into the plate, allowing for colonies to form at different depths within the agar.
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You can have insurance on a suspended plate. If you get in an accident with a suspended plate, though, your insurance company may not cover the accident because the car was not legal to drive.
Microbe colonies develop in larger sizes on sparsely seeded plates due to the abundance of plate surface they have for growth. Heavily seeded plates produce smaller colonies as they are forced to compete with one another for basic survival.
The purpose of a pour plate is to determine the concentration of bacteria in a sample by counting the number of colonies that grow on the agar plate after incubation. This method allows for both surface and subsurface colonies to be counted, providing a more accurate representation of the bacterial population in the sample.
The molten material in a plate is called “magma.” Magma is a mixture of molten rock, suspended mineral crystals, and dissolved gases beneath the Earth's surface. If magma reaches the surface and flows out, it is then referred to as lava.
The process of applying a specimen to an agar plate to grow colonies is known as streaking. This technique involves using an inoculating loop to spread the specimen across the surface of the agar in a pattern that promotes the isolation of individual colonies for further study.
When performing a streak plate culture, the nutrient plate should be rotated in a clockwise or counterclockwise direction to create isolated colonies on different parts of the plate. The rotation ensures that the inoculating loop spreads the bacteria evenly across the agar surface, leading to distinct colonies for further analysis.
Because if the plates are wet you will not get individual colonies, instead you will get a film of bacteria growing in the water film on the surface of the plate. This can ruin a selection for transformants as the antibiotic will not be present in the water film on the surface of the plate.