How do restriction enzymes create recombinant DNA?

Answer 1

There are many methods, though one of the most common is the use of restriction endonucleases. These enzymes can be used to cut DNA fragments at specific locations. Cut DNA fragments will recombine into new orders, which are sealed using DNA ligase. A selection process must be used to locate the desired recombinant DNA, since it will be in a mixture of various undesired recombinations.

Answer 2

The gene to be replicated is inserted into copies of a plasmid containing genes that make cells resistant to particular antibiotics and a multiple cloning site (MCS, or polylinker), which is a short region containing several commonly used restriction sites allowing the easy insertion of DNA fragments at this location. Next, the plasmids are inserted into bacteria by a process called transformation. Then, the bacteria are exposed to the particular antibiotics. Only bacteria that take up copies of the plasmid survive, since the plasmid makes them resistant. In particular, the protecting genes are expressed (used to make a protein) and the expressed protein breaks down the antibiotics.

Answer 3

Recombinant DNA is prepared by choosing a vector and and a genome. After selection genomic DNA is cut with the help of Restriction enzyme like BamHI or EcoRI and then this specific DNA is integrated in to the vector like plamid vector or pBR322 and this integrated is transfer in to the host cell for further production of recombinant colonies or cell.

Answer 4

The DNA sequence(gene of interest) first be cutted off from the genome. For that

if, it is a whole genome, it was mechanically sheared, and electrophoresed and identified by combination od southern blotting and hybridization.

Then the gene is ligated into the vector(plasmid),

before that, the plasmid which can be in closed circular form will be converted to open form, by adding restriction enzymes appropriate to that.,

Then the gene will be inserted into the plasmid with E.Coli or T4 lambda phage ligase.

Now the plasmid will be transformed into a host organism..!

Answer 5

Restriction enzymes are used to cut DNA into fragments. This allows the plasmid to be placed in the gaps created from the cut DNA. Recombinant DNA, therefore, is DNA from two different sources that would not usually bind together, and is made artificially.

Answer 6

Recombinant DNA is a type of artificial DNA.

It made by combiing two or more Sequences that wouldn't normally occur together through gene splicing.