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The process of gram staining is simple.
1)smear bacteria from pure culture onto slide, heat fix
2)flood with crystal violet (1min)
3)Add iodine (1 min)
4)acid/alcohol wash (1 min)
5)Flood with safranine (1min)
6)Air dry and examine.
These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close).
Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer.
Source(s):
Medical Microbiology
What else can I help you with?
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B. Subtilus is a rod or bacilus shaped, gram positive bacteria
Eliminate gram-positive bacteria from a mixture of gram and gram-bacteria which procedure would be best 1treatment with mild detergent or lysozyme?
to eliminate gram positive bacteria from a mixture of grampositive and gram negative bacteria which procedure would be best first treatment with mild detergent or lysoyme
Does the presence of nuclei in fungi as well as its ability to reproduce asexually distinguish from bacteria?
Those are two of many characteristics that distinguish fungi from bacteria.
How did scientists produce oil eating bacteria?
Inducing mutations in bacteria
Why did scientists place bacteria in their kingdom the monera?
Scientists placed bacteria in their own kingdom, the Monera, because bacteria lack the nuclei, mitochondria, and chloroplasts found in other forms of life
Scientists use bacteria to produce?
Insulin-producing bacteria were commercialized in 1982.
What type of scientists study bacteria?
A microbiologist.
Why did the scientists place bacteria in their own kingdom the Monera?
Scientists placed bacteria in their own kingdom, the Monera, because bacteria lack the nuclei, mitochondria, and chloroplasts found in other forms of life
What characteristics distinguish protists from bacteria?
Protists have nucleus and double membranous organells.Bacteria do not have them.
How have scientists made bacteria more useful today?
Scientists have taken restriction enzymes out of bacteria; restriction enzymes are used to cut DNA at cut sites. Also, they insert genes into bacteria to study them.
What two kingdoms do scientists divide bacteria in to?
Scientists divide bacteria into two kingdoms: Bacteria (also known as Eubacteria) and Archaea. These two kingdoms are based on differences in their genetic and biochemical makeup.
How do scientists classify bacteria?
Scientists classify bacteria based on their shape, structure, biochemical properties, and genetic composition. Bacteria are classified into different groups, such as phyla, classes, orders, families, genera, and species, using a system called taxonomy. This classification helps scientists understand the diversity and relationships among different bacteria species.
