the pH of ammonium acetate buffer can be adjusted with glacial acetic acid (concentrate or diluted )
To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Adjust 1 L of this solution to pH 4.5 by adding acetic acid (about 8 ml) and 5 ml of 1 M p-TSA (equivalent to 5 mM p-TSA).
Dissolve 25g of Ammonium acetate in 25ml of water and add 38ml of 7M hydrochloric acid. Adjust the pH of the solution to 3.5 with either 2M hydrochloric acid or 6M ammonia and dilute with water to 100ml
can I make sodium acetate buffer 0.2M Ph=5 whit sodium acetate buffer 3m Ph=5 solution?
It depends on the molarity of the solution
it because of the ammonium ion form in the solution. Ammonium solution is an alkaline.
To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Adjust 1 L of this solution to pH 4.5 by adding acetic acid (about 8 ml) and 5 ml of 1 M p-TSA (equivalent to 5 mM p-TSA).
Dissolve 25g of Ammonium acetate in 25ml of water and add 38ml of 7M hydrochloric acid. Adjust the pH of the solution to 3.5 with either 2M hydrochloric acid or 6M ammonia and dilute with water to 100ml
pH of 0.1M solution is 5.5.
can I make sodium acetate buffer 0.2M Ph=5 whit sodium acetate buffer 3m Ph=5 solution?
The water solution of sodium acetate has a basic pH.
It depends on the molarity of the solution
4
The solution is acidic.
it because of the ammonium ion form in the solution. Ammonium solution is an alkaline.
Explosion A slight amount of chlorine gas could result (though not likely), otherwise Ammonium Acetate & Water in a stable form. Ammonium Acetate is sometimes used as a decent biodegradable de-icing agent.
The question is in poorly worded. I will assume the question is "why adjust the pH of Tris buffer with HCl and not Sodium Acetate?" I would assume the answer is - because sodium acetate is the conjugate base of a weak acid, and HCl is a strong acid. Also the salts you would be putting into the solution as a result would be different. I think the question is actually, "The pH of Tris is adjusted with HCl, why isn't the pH of sodium acetate adjusted with HCl?". I'm not sure of the answer exactly, but I've always assumed its because if you adjust the pH with glacial acetic acid instead of HCl, you won't introduce chloride ions.
ist combination Acetic acid and Sodium acetate pH acidic, 2nd combination Ammonium hydroxide and Ammonium chloride pH basic.