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How do you calculate percent inhibition?
% inhibition = [(normal activity - inhibited activity) / (normal activity)] * 100%
What switchs on enzyme activity while what can switch off or reduce enzyme activity?
activators; inhibitors
What can change the way an enzyme acts?
Enzyme activity is affected by other molecules, temperature, chemical environment (e.g., pH), and the concentration of substrate and enzyme. Activators are molecules that encourage enzyme activity, and inhibitors are enzymes that decrease enzyme activity. Sometimes a cofactor is necessary for the enzyme to work.
What accurately describes how temperature affects enzyme activity?
Enzyme activity increases with temperature, but only up to a point.
What determine an enzymes activity?
There are several things that determine an enzyme's activity. The main determinants include the structure of the enzyme, temperature, pH and so much more.
How do you calculate specific activity of salivary amylase?
Divide the rate of enzyme activity by the protein concentration.
How do you calculate enzyme enrichment?
Enrichment=specific activity of fraction/original specific activity of original sample
How do you calculate percent inhibition?
% inhibition = [(normal activity - inhibited activity) / (normal activity)] * 100%
What can you do to regain the activity of the enzyme?
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
What is the effect of hydrochloric acid on the enzyme activity?
Denature enzyme activity
What are the effects of physical activity on enzyme activity?
Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive
What are the effects of freezing on enzyme activity?
When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.
What switchs on enzyme activity while what can switch off or reduce enzyme activity?
activators; inhibitors
What is the effect of cold temperatures on enzyme activity?
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
What can change the way an enzyme acts?
Enzyme activity is affected by other molecules, temperature, chemical environment (e.g., pH), and the concentration of substrate and enzyme. Activators are molecules that encourage enzyme activity, and inhibitors are enzymes that decrease enzyme activity. Sometimes a cofactor is necessary for the enzyme to work.
Which blocks enzyme activity by binding to the site of an enzyme?
inhibitor
What accurately describes how temperature affects enzyme activity?
Enzyme activity increases with temperature, but only up to a point.
