purity testing of microorganism is to check weahter the microbial culture contain one type of cells of species or it get contaminated or mixed with other type.the best way to check this is by sub culturing and staining.
A normal culture may be contaminated by a mixture of microorganisms normally found on a person's skin (normal flora).
A mixed culture is a container that holds two or more identified and easily differentiated species of microorganisms. A contaminated culture was once pure or mixed (and thus a known entity) but since had contaminants (unwanted microbes of uncertain identity) introduced into it.
If a culture of salmonella is left open to the air it can become contaminated with other airborne bacteria.
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The bacteria on the margin are the most active and healthiest because they have access to fresh nutrients and have not yet contaminated the culture medium with waste.
Contamination of culture can be indicated by changes in the appearance, smell, or pH of the culture medium, growth of unintended microorganisms, or unexpected outcomes in experiments that use the culture. Turbidity, color changes, or presence of particulate matter can also suggest contamination. Regularly monitoring cultures and conducting controls can help in detecting contamination.
The rods will be gram negative, the cocci are gram positive: most likely, the slide was made from a mixed culture of bacteria, or the culture was contaminated.
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The water supply was contaminated. The company contaminated the soil with their chemicals.
Definitely not. However, blood cultures can be contaminated, and further blood cultures should be done to exclude contamination.