How do you describe transformation in bacteria?
When bacteria incorporate foreign DNA into their own best describes transformation in bacteria. An example would be the infection of cells by a phage DNA molecule.
Briefly describe how Avery and his group determined which molecule was most important for transformation?
Avery and his colleagues made an extract, or juice, from the heat-killed bacteria. They then carefully treated the extract with enzymes that destroyed proteins, lipids, carbohydrates, and other molecules, including the RNA. Transformation still occurred. Obviously, since these molecules had been destroyed, they were not responsible for the transformation. Your welcome:)
What one plate would you first inspect to conclude that the transformation occurred successfully Why?
If you transform bacteria with a plasmid containing a selection marker (such as an antibiotic resistance gene) and plate the transformed bacteria on a plate suited for selecting for plasmid-containing bacteria (such as a plate containing an antibiotic that only those bacteria with antibiotic resistance can survive), then simply inspecting whether colonies are present on the plate will suffice in determining whether the transformation succeeded. If no colonies are found, that means no bacteria got…
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells