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How do you determine the pKa value of an organic compound for HPLC method?
Wiki User
∙ 13y ago
the organic compound three kind normal, acid and basic compound for acid compound the pKa from 3 to 5, normal pKa around 7 and basic compound more than 7
also you can know the kind of organic compound from structure that you can see acidic or basic grope like carboxylic acid or amine.
Wiki User
∙ 13y ago
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How you find out Pka value of organic compound for HPLC method develoment?
If you can't find it in the literature it can be determined experimentally by titration.
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Use a method called titration, where a known concentration of either a strong acid (to measure a weak base) or a strong base (to measure a weak acid) is slowly added to a solution of the unknown compound. By measuring the pH of the solution as you do the titration, and by measuring the volume of the known solution that you added to the unknown, you can determine the pKa of the unknown compound.For complete instructions on how a titration is performed, see the Web Links to the left of this answer.how can i determine pka value if pH is given?
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the volatility of any unknown compound can be known by comparison method between the compound whose volatility is known by the use of paper chromatography. since the high volatile compound has less or no adsorptions to the stationary phase of the parer (the compound is less polar or non-polar) it will move high with the solvent, thus, having high Rf value. The polar compound or less volatile compound will have less Rf value. The conclusion therefore, can be made from the differences in their Rf values
