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How do you find antibiotic assay?

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Anonymous

14y ago
Updated: 8/19/2019

For determining the type of causal agent of Infectious Diseases and for checking their sensitivity to antibiotics and chemotherapeutic agents in vitro by means of the inhibition zone determination method. The antibiogram allows rational and selective chemotherapy. The test discs can be coated with chemotherapeutic agents, placed on the innoculated nutrient agar and incubated. The size of the inhibition zone is a measure for the effectiveness of the substances.

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Wiki User

14y ago

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Is pyrogen test an antimicrobial assay?

No. Pyrogen testing is a process used by drug manufacturers to see if a vaccine or drug has toxins that might cause a fever.An antibiotic assay is used to determine the potency of the antibiotic.


What method is used to test the effectiveness of 2 different antibiotics?

One common method is the disc diffusion assay, where paper discs saturated with each antibiotic are placed on a bacterial lawn, and the size of the resulting inhibition zone is measured. Another method is the broth microdilution assay, where different concentrations of each antibiotic are tested in liquid culture to determine the minimum inhibitory concentration (MIC) required to stop bacterial growth.


How does the antibiotic get from the disk tnto the agar?

The antibiotic in a disk diffusion assay diffuses from the disk into the agar medium due to a concentration gradient. As the antibiotic moves away from the disk, it spreads out into the surrounding agar, creating a zone of inhibition where bacterial growth is prevented. The rate of diffusion depends on factors like the size of the antibiotic molecule and the agar's characteristics. This process allows researchers to assess the antibiotic's effectiveness against specific bacteria by measuring the diameter of the inhibition zone.


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What are the limitations of the disk diffusion assay?

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When did US Assay Commission end?

United States Assay Commission ended in 1980.


Can you put the word assay in a sentence?

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