follow this procedure.. 1.Collect two onion root tips and trim so that you have about 2 mm of the very tip. 2.Soak the tips in a few drops of 1 M HCl in a watch glass for about 10 min. 3.Transfer the tips to a few drops of acetocarmine stain for about 15 min. 4.Transfer one tip to a few drops of water on a slide. Lower a cover slip onto the tip and press it down gently so that the tip squashes out to about 7 mm (1/3 inch) around. 5.Look for mitoses under high power (X40) or under oil immersion (X100) (If this fails for any reason try your other root tip)
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Onion root tips
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The time of cell division usually relates to the life span of a plant or animal. For onion root tips, the length of the cell division is related to the life span of the onion plant.
The reason for mitosis occurring most commonly in the onion root tip is because the onion root tip is a rapidly growing area of the plant.
20, 10, 3, 2, 1 = 365, 10, 33.3, 50, 1.7 =100%?, ?, ?, ?, ?, ?= 24
Iodine, its yellow and stains cells very well for viewing under a microscope.
On the tips of the root
Roots hairs absorb water and minerals,and protect root tips.
Video: How do you dice an onion?The most important thing about dicing an onion is to breathe through your mouth! This will keep you from tearing up while dicing.The first thing you want to do is cut the top off of your onion (NOT the root end). After you cut off the top, then you can grab the edge and peel off the skin of the onion.Hold the onion firmly, but tuck the tips of your fingers inward as not to cut them with your knife. The next thing you are going to do is make parallel cuts on your onion, slicing from the top to the root without cutting through the root. You do NOT want to slice all the way through the onion. Make several parallel slices from top to bottom, getting as close to the root as possible. Make your parallel cuts as far apart as you want your diced pieces to be.Next, turn your onion 90 degrees, and make another series of parallel cuts (from top to root without cutting through) perpendicular to the cuts you already made, so your onion looks like a grid.Then, holding your onion pieces together, place the onion on its side, and start making downward slices.You now have a diced onion!
The acid hardens the root tip so that it is less likely to break. The root tips have to be heated so that the stain is up taken and able to properly reach the chromatin ( which is dyed by the acectic orecin stain). This helps the cells to be seen more clearly under the microscope.
root cap