You will need to remove it.
consider using waterproofing before applying you next silicone coat then use Siliconized water based elastomeric acrylic.
you can find it here http://www.tiledepot.us/setting-materials-n-more-83/grout-caulk-thinset-n-more-142/c-cure-caulk-non-sanded-506.html
Mildew may be cleaned from bathtub caulk and bathroom walls the same way. If the mildew extends below the caulk, however, there is no point in cleaning it; it will soon return. Better to remove the old caulk and apply new. For walls, there is less chance for mildew to develop if the walls are painted with a high gloss finish rather than matte. For tile grout, try using a commercial spray-on mold and mildew remover. Run the bathroom exhaust fan while taking showers or running the bath to slow the development of mildew.
The most effective method is to wash the affected surfaces with a chlorine bleach, that will kill the mildew. Wear a rubber glove!
Bleach will safely remove any type of mold or mildew stains.
Most shower stains are either mildew or soap scum (which is a combination of product and lime build up). If I start getting stains in the shower, I use comet to scrub the tub down. Let it sit for a while, and then rinse it out very thoroughly. When you are finished, if the stains are still there, try Lime-A-Way. It also works on your shower head! Black stains on tile are typically caused by mildew, an air-borne fungus that thrives in humid locations. Use a weak concentration of chlorine bleach in a spray bottle, but make sure the shower gets plenty of air when you do that, the chlorine can cause you somee problems if it gets too strong. Use 5% to 10% chlorine bleach in water and spray it directly on the mildew. If it dries out too quickly, you may need to use some tissue to keep it wet longer. Spray the mixture on the tissue and bunch up the wet tissue over the stains. If the stains are white, brown or green or basically any color except black, it might be mineral stains and you'll need to use something like CLR to get the stuff off.
no
with an acidic substance, such as rubbing alcohol
physical change
You get rid of oil stains by using oxce clean
I read that rubbing alcohol will do the trick. Heres the link. http://home.howstuffworks.com/how-to-remove-mildew-stains.htm
You will always have to change your curtain once in a while, but heavier vinyl shower curtains resist mildew more. Amazon sells these curtains for a fair price, and they're very nice. They will last you at least a year, if that. http://www.amazon.com/Mold-Mildew-Resistant-Shower-Curtain/dp/B0002U37GM
Bit of spit and a rub
I would use a white mildew resistant shower curtain liner behing the Ruffles shower curtain.
There should be no need to destroy vital evidence of a crime.
scrub it