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You can differentiate between different salmonella strains using serology. Each strain has its own combination of antigens. Depending on the O, H, and vi antigens, you can determine which strain is present. The O-antigen is the somatic polysaccharide antigen, the H-antigen is the flagellar antigen and the vi-antigen is the capsular antigen. Antibodies to most of the variants of these antigens are available commercially for use in labs. Adding an aliquot of specific antisera to a suspension of your salmonella spp, and incubating it for a few hours in a water bath will result in visible agglutination if your salmonella strain possesses the specific antigen to the antibody you added. For example, Salmonella typhi will show agglutination with O [9,12], H [d] and vi.

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Q: How do you identify different Salmonella species by biochemical test?
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