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How do you make 0.02M sodium phosphate buffer of pH 6.9 with 0.006M NaCl?
Updated: 8/10/2023
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∙ 8y agoBest Answer
Prepare 0.02M Na2HPO4 (di-basic) and 0.02M NaH2PO4 (mono-basic) separately, make up the volume for both. Check the pH for both separately. Add the one that has lower pH to the one that has a higher pH until the pH reaches 6.9. Add 0.006M Nacl.
What amount of 0.006M to be added?
Wiki User
∙ 8y agoThis answer is:
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Wiki User
∙ 16y agothere is a special website for these calculations.. so please try them http://www.columbia.edu/~scb2001/tools/phosphate/phosphate.html
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