Make Solution A by dissolving 174.18g of K2HPO4 in 1L of dH2O.
Make solution B by dissolving 136g of KH2PO4 in 1L of dH2O.
now mix solution A and B and finally adjust pH of your buffer.
Dibasic potassium phosphate has the chemical formula is KH2PO4. The standardized solutions has a pH between 6 and 8.
Add 250 ml of 0.2M KH2PO4 to 393.4 ml of 0.1M NaOH
pH range of KH2PO4 is approx 4.3-4.7
KH2PO4 is made up of potassium dihydrogen phosphate. Some examples of weak acids are water and carbon dioxide, ethanic acid and ammonia.
inorganic
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Dibasic potassium phosphate has the chemical formula is KH2PO4. The standardized solutions has a pH between 6 and 8.
Add 250 ml of 0.2M KH2PO4 to 393.4 ml of 0.1M NaOH
pH range of KH2PO4 is approx 4.3-4.7
KH2PO4 is made up of potassium dihydrogen phosphate. Some examples of weak acids are water and carbon dioxide, ethanic acid and ammonia.
inorganic
pH mL 1M K2HPO4 mL KH2PO4 (mL) 5.8 8.5 91.5 6.0 13.2 86.8 6.2 19.2 80.8 6.4 27.8 72.2 6.6 38.1 61.9 6.8 49.1 50.3 7.0 61.5 38.3 7.2 71.7 28.3 7.4 80.2 19.8 7.6 86.6 13.4 7.8 90.8 9.2 8.0 94.0 6.0
Kh2po4
Yes, it is an inorganic compound.
KH2PO4=136 weigh 68g dissolve in 1 liter of water
H2CO3 is not used as buffer.
Molarity of 1X PBS is 10mM. To prepare 20mM PBS take 16g of NaCl, 0.4 g KCl, 2.88 g Na2HPO4 0.48 g KH2PO4 in 800 ml of distilled H2O. Adjust the pH to 7.4 with HCl. Add H2O to 1 liter.