How do you make semi solid media?

Answer 1

Solid media is nutrient medium on whcih, when streak with the bacterial sample the bacterial colonies developed. It is commonly used to isolate different bacterial species or to have a pure culture i.e. a culture containing only one type of microorganism. At first R. Koch a German physician used to grow bacterial colony on boiled and sliced potatoes. Eventually he developed the culture media using meat extracts and protein digests and solidify it by using gelatin. Although Gelatin is consumed by many bacterial species. Due to this disadvantage of gelatin Fannie Elishemius Hesse, the wife of Walther Hesse, one of the Koch's assistants suggest using AGAR as solidifying agent. Agar was not attacked by most bacteria and did not melt until reaching a temprature of 100oC. Furthermore once melt it did not solidify until it reached a temprature of 50oC.

Answer 2

To make semi-solid media, mix agar or gelatin into the liquid growth medium while heating until dissolved. Then pour the mixture into Petri dishes or test tubes and allow it to cool and solidify. The final product will be a semi-solid substance that can support the growth of microorganisms.

Answer 3

a semi-solid media is a media which contain a small amount of agar {around 0.4%} to make it much soft, the agar is sufficiently soft which allow the motile bacteri to swim.