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Molecular weight of EDTA disodium salt dihydrate: 372.24 grams/mole
This molecular weight is used for preparation is because most hydrate salts are easier to dissolve in solution rather than anhydrous salts.
0.1N EDTA
250mL solution
Normality is described by how many equivalents of protons are available to participate in your reaction. For example, strong acids like a 1M H2SO4 automatically deprotonate in water to release two protons per one molecule of H2SO4, which means a 1M H2SO4 = 2N H2SO4.
EDTA has the ability to deprotonate 4 times, but it does NOT all deprotonate the moment it hits the solution. It's pkas are approximately ~2, 3, 6, 10. If your solution is to be at pH = 8, then you will have 3 equivalents of protons released into the solution (pkas of 2,3,6 are more than 1 unit lower than pH = 8, so protons are released). If it is at pH = 5, then you will release 2 equivalents. Most molecular Biology work working with enzymes near neutral pH will buffer EDTA at pH = 8. However, since this question is asking for a preparation of EDTA without a buffering effect, I will assume all four protons will be used.
(0.1N EDTA) x (1M equivalent Na2EDTA.2H2O/ 4 N equivalent protons ) x (1L/1000ml) x (250ml solutionl) x (372.24 grams/ mole) = 2.33 grams Na2EDTA.2H2O required.
Dissolve 2.33 grams Na2EDTA.2H2O into 150ml ddH2O, increase pH to 8 with NaOH pellets and maintain this pH until all EDTA is dissolved. Add more NaOH pellets to pH 12, then bring volume up to 250ml withddH2O.
What else can I help you with?
Is EDTA is water soluble if not than how will you prepare your solution?
Yes, EDTA is water-soluble. To prepare a solution, you can simply add the desired amount of EDTA powder to water and stir until it is completely dissolved. If you encounter issues with solubility, you can adjust the pH of the solution using sodium hydroxide or hydrochloric acid to help dissolve the EDTA.
How do you prepare 5mM EDTA of 100ml solution?
5mM = 0.005 moles 100 mL = 0.1 Liters Molarity = moles of solute/Liters of solution 0.005 M EDTA = X moles/0.1 Liters = 0.0005 moles EDTA =_____________ Now, look up the molecular formula for EDTA and find how many grams needed to add to your 100 mL.
How you can do EDTA titration reaction?
To perform an EDTA titration, first prepare a solution containing the analyte (the substance being measured) and a suitable indicator, such as Eriochrome Black T. Add a standardized solution of EDTA to the analyte solution until the endpoint is reached, indicated by a color change in the indicator. The volume of EDTA solution added can be used to calculate the concentration of the analyte based on the stoichiometry of the reaction.
How do you prepare 1mM EDTA from 0.1M EDTA?
0.1M is 1/10 molar whereas 1mM is 1 millimolar and thus 1/1000 molar. There is thus a 1:100 dilution. So 10:1000 would be the same. To a 1000ml volumetric flask, pipete 10mls of 0.1M EDTA solution. Make up to the mark with deionized water. Mix and shake and you will have 1000mls of 1mM EDTA solution.
How can you prepare 100mM EDTA?
To prepare 100mM EDTA solution, dissolve 37.2g of EDTA disodium salt dihydrate in 1 liter of water. Make sure the pH is adjusted to around 8.0 with sodium hydroxide or hydrochloric acid if needed. Mix well until EDTA is fully dissolved.
How much gm of disodium edta require to prepare 0.05 m disodium edta solution?
To prepare a 0.05 M disodium EDTA solution, you would need to dissolve 3.72 grams of disodium EDTA dihydrate (Na2C10H14N2Na2·2H2O) in enough water to make 1 liter of solution.
Is EDTA is water soluble if not than how will you prepare your solution?
Yes, EDTA is water-soluble. To prepare a solution, you can simply add the desired amount of EDTA powder to water and stir until it is completely dissolved. If you encounter issues with solubility, you can adjust the pH of the solution using sodium hydroxide or hydrochloric acid to help dissolve the EDTA.
How do you prepare EDTA N50 solution?
for 1 leter- dissolve 3.7225 gm EDTA in 1 leter boild out disttiled water
How you can do EDTA titration reaction?
To perform an EDTA titration, first prepare a solution containing the analyte (the substance being measured) and a suitable indicator, such as Eriochrome Black T. Add a standardized solution of EDTA to the analyte solution until the endpoint is reached, indicated by a color change in the indicator. The volume of EDTA solution added can be used to calculate the concentration of the analyte based on the stoichiometry of the reaction.
How do you prepare 5mM EDTA of 100ml solution?
5mM = 0.005 moles 100 mL = 0.1 Liters Molarity = moles of solute/Liters of solution 0.005 M EDTA = X moles/0.1 Liters = 0.0005 moles EDTA =_____________ Now, look up the molecular formula for EDTA and find how many grams needed to add to your 100 mL.
How do you prepare 1mM EDTA from 0.1M EDTA?
0.1M is 1/10 molar whereas 1mM is 1 millimolar and thus 1/1000 molar. There is thus a 1:100 dilution. So 10:1000 would be the same. To a 1000ml volumetric flask, pipete 10mls of 0.1M EDTA solution. Make up to the mark with deionized water. Mix and shake and you will have 1000mls of 1mM EDTA solution.
How can you prepare 100mM EDTA?
To prepare 100mM EDTA solution, dissolve 37.2g of EDTA disodium salt dihydrate in 1 liter of water. Make sure the pH is adjusted to around 8.0 with sodium hydroxide or hydrochloric acid if needed. Mix well until EDTA is fully dissolved.
How much quantity of EDTA dissolved in 1000ml to get 0.01 m solution of EDTA?
To prepare a 0.01 M solution of EDTA in 1000 ml, you would need 37.22 grams of EDTA disodium salt dihydrate (C10H14N2Na2O8·2H2O) or approximately 0.1 moles. Dissolve the EDTA in water and make up the volume to 1000 ml to get a 0.01 M solution.
How do you can prepare 0.5molar EDTA solution?
To prepare a 0.5 M EDTA solution, dissolve the appropriate amount of EDTA disodium salt dihydrate (molecular weight 372.24 g/mol) in water to achieve a final volume desired. For example, to make 100 mL of 0.5 M EDTA solution, you would dissolve 18.61 g of EDTA disodium salt dihydrate in water and adjust the volume to 100 mL.
How many grams of EDTA do you add to make a 3.7 percent EDTA solution?
To make a 3.7% EDTA solution, you would add 3.7 grams of EDTA to 100 mL of solution.
How do you prepare 1mM edta from 0.1M edta-Na2 solution?
You dilute it 1:10, then you take 1 part of that solution and mix it with 9 parts of the diluent. That will make the 1:100 dilution you need, incl. prevention of pipette inaccuracy.
When edta is insoluble in distilled water then how to make 0.01N edta solution?
use heat to heat the solution and add EDTA slowly to dissolve it.
