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Molecular weight of EDTA disodium salt dihydrate: 372.24 grams/mole
This molecular weight is used for preparation is because most hydrate salts are easier to dissolve in solution rather than anhydrous salts.
0.1N EDTA
250mL solution
Normality is described by how many equivalents of protons are available to participate in your reaction. For example, strong acids like a 1M H2SO4 automatically deprotonate in water to release two protons per one molecule of H2SO4, which means a 1M H2SO4 = 2N H2SO4.
EDTA has the ability to deprotonate 4 times, but it does NOT all deprotonate the moment it hits the solution. It's pkas are approximately ~2, 3, 6, 10. If your solution is to be at pH = 8, then you will have 3 equivalents of protons released into the solution (pkas of 2,3,6 are more than 1 unit lower than pH = 8, so protons are released). If it is at pH = 5, then you will release 2 equivalents. Most molecular Biology work working with enzymes near neutral pH will buffer EDTA at pH = 8. However, since this question is asking for a preparation of EDTA without a buffering effect, I will assume all four protons will be used.
(0.1N EDTA) x (1M equivalent Na2EDTA.2H2O/ 4 N equivalent protons ) x (1L/1000ml) x (250ml solutionl) x (372.24 grams/ mole) = 2.33 grams Na2EDTA.2H2O required.
Dissolve 2.33 grams Na2EDTA.2H2O into 150ml ddH2O, increase pH to 8 with NaOH pellets and maintain this pH until all EDTA is dissolved. Add more NaOH pellets to pH 12, then bring volume up to 250ml withddH2O.
Trever Windler
Kevin Greenfelder
You mast to first accurate wight 3.7224 ,then transfere to flask mayer add 400 ml water dianoes until dissolve then volume with water to marl 1 liter.
Wiki User
∙ 14y agoMolecular weight of EDTA disodium salt dihydrate: 372.24 grams/mole
This molecular weight is used for preparation is because most hydrate salts are easier to dissolve in solution rather than anhydrous salts.
0.1N EDTA
250mL solution
Normality is described by how many equivalents of protons are available to participate in your reaction. For example, strong acids like a 1M H2SO4 automatically deprotonate in water to release two protons per one molecule of H2SO4, which means a 1M H2SO4 = 2N H2SO4.
EDTA has the ability to deprotonate 4 times, but it does NOT all deprotonate the moment it hits the solution. It's pkas are approximately ~2, 3, 6, 10. If your solution is to be at pH = 8, then you will have 3 equivalents of protons released into the solution (pkas of 2,3,6 are more than 1 unit lower than pH = 8, so protons are released). If it is at pH = 5, then you will release 2 equivalents. Most molecular Biology work working with enzymes near neutral pH will buffer EDTA at pH = 8. However, since this question is asking for a preparation of EDTA without a buffering effect, I will assume all four protons will be used.
(0.1N EDTA) x (1M equivalent Na2EDTA.2H2O/ 4 N equivalent protons ) x (1L/1000ml) x (250ml solutionl) x (372.24 grams/ mole) = 2.33 grams Na2EDTA.2H2O required.
Dissolve 2.33 grams Na2EDTA.2H2O into 150ml ddH2O, increase pH to 8 with NaOH pellets and maintain this pH until all EDTA is dissolved. Add more NaOH pellets to pH 12, then bring volume up to 250ml withddH2O.
To prepare a 0.02N EDTA solution, dissolve 3.72 grams of EDTA disodium salt dihydrate (C10H14N2Na2O8·2H2O) in 1 liter of distilled water. Ensure that the pH of the solution is adjusted to around 8-9 using a suitable buffer.
Wiki User
∙ 12y ago3.8 grams of EDTA salt in 1 liter of DI water made up using a volumetric flask will give you 0.02n or 0.01m of EDTA solution.
normality*eq.wt*volume rqrd
weight= 1000
then will get weight of the compound required for that normality
Wiki User
∙ 11y ago1. Weigh 5,8448 g of EDTA.
2. Quantitatively transfer EDTA in a clean and dry volumetric flask of 1L.
3. Add 900 mLof demineralized water.
4. Place the the volumetric flask in a thermostat at 20 0C.
5. Wait half hour.
6. Add demineralized water to the mark.
7. Put a glass stopper and stir the content.
8. Add a label with: data, name of the solution, concentration of the solution, operator.
Wiki User
∙ 7y agoThe molar mass of EDTA is 292.24 g/mol. To make 1 liter of 0.01 N EDTA proceed as follows, considering that 0.01 N means 0.01 equivalents/liter and there are 4 equivalent wts/mole.
0.01 equiv/L x 1 L x 1 mol/4 equiv x 292.24 g/mole = 0.73 grams EDTA in a final volume of 1 liter.
Wiki User
∙ 11y agoYou mast to first accurate wight 3.7224 ,then transfere to flask mayer add 400 ml water dianoes until dissolve then volume with water to marl 1 liter.
Wiki User
∙ 10y agoDissolve 5,8448 g EDTA in 0,9 L demineralized water in a clean volumetric flask. At 20 0C add water to the mark and stir.
Wiki User
∙ 6y agoDissolve 58,448 g dried EDTA in distilled water using a volumetric flask.
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Is EDTA is water soluble if not than how will you prepare your solution?
Yes, EDTA is water-soluble. To prepare a solution, you can simply add the desired amount of EDTA powder to water and stir until it is completely dissolved. If you encounter issues with solubility, you can adjust the pH of the solution using sodium hydroxide or hydrochloric acid to help dissolve the EDTA.
How do you prepare 5mM EDTA of 100ml solution?
To prepare a 5mM EDTA solution in 100ml, you would need to weigh out 0.375g of EDTA (molar mass of EDTA is 292.24 g/mol) and dissolve it in distilled water to make up a final volume of 100ml. Make sure to use a balance to accurately measure the EDTA.
How you can do EDTA titration reaction?
To perform an EDTA titration, first prepare a solution containing the analyte (the substance being measured) and a suitable indicator, such as Eriochrome Black T. Add a standardized solution of EDTA to the analyte solution until the endpoint is reached, indicated by a color change in the indicator. The volume of EDTA solution added can be used to calculate the concentration of the analyte based on the stoichiometry of the reaction.
How do you prepare 1mM EDTA from 0.1M EDTA?
To prepare 1mM EDTA from 0.1M EDTA, you would dilute the 0.1M EDTA solution 100-fold. This means you would mix 1 volume of 0.1M EDTA with 99 volumes of a solvent (such as water) to reach a final concentration of 1mM EDTA.
How can you prepare 100mM EDTA?
To prepare 100mM EDTA solution, dissolve 37.2g of EDTA disodium salt dihydrate in 1 liter of water. Make sure the pH is adjusted to around 8.0 with sodium hydroxide or hydrochloric acid if needed. Mix well until EDTA is fully dissolved.
Is EDTA is water soluble if not than how will you prepare your solution?
Yes, EDTA is water-soluble. To prepare a solution, you can simply add the desired amount of EDTA powder to water and stir until it is completely dissolved. If you encounter issues with solubility, you can adjust the pH of the solution using sodium hydroxide or hydrochloric acid to help dissolve the EDTA.
How do you prepare EDTA N50 solution?
for 1 leter- dissolve 3.7225 gm EDTA in 1 leter boild out disttiled water
How do you prepare 1mM edta from 0.1M edta-Na2 solution?
You dilute it 1:10, then you take 1 part of that solution and mix it with 9 parts of the diluent. That will make the 1:100 dilution you need, incl. prevention of pipette inaccuracy.
How do you prepare 5mM EDTA of 100ml solution?
To prepare a 5mM EDTA solution in 100ml, you would need to weigh out 0.375g of EDTA (molar mass of EDTA is 292.24 g/mol) and dissolve it in distilled water to make up a final volume of 100ml. Make sure to use a balance to accurately measure the EDTA.
How do you can prepare 0.5molar EDTA solution?
To prepare a 0.5 M EDTA solution, dissolve the appropriate amount of EDTA disodium salt dihydrate (molecular weight 372.24 g/mol) in water to achieve a final volume desired. For example, to make 100 mL of 0.5 M EDTA solution, you would dissolve 18.61 g of EDTA disodium salt dihydrate in water and adjust the volume to 100 mL.
When edta is insoluble in distilled water then how to make 0.01N edta solution?
use heat to heat the solution and add EDTA slowly to dissolve it.
How do you prepare 1mM EDTA from 0.1M EDTA?
To prepare 1mM EDTA from 0.1M EDTA, you would dilute the 0.1M EDTA solution 100-fold. This means you would mix 1 volume of 0.1M EDTA with 99 volumes of a solvent (such as water) to reach a final concentration of 1mM EDTA.
How can you prepare 100mM EDTA?
To prepare 100mM EDTA solution, dissolve 37.2g of EDTA disodium salt dihydrate in 1 liter of water. Make sure the pH is adjusted to around 8.0 with sodium hydroxide or hydrochloric acid if needed. Mix well until EDTA is fully dissolved.
How do you prepare 0.02N solution?
3.8 grams of EDTA salt in 1 liter of DI water made up using a volumetric flask will give you 0.02n or 0.01m of EDTA solution. normality*eq.wt*volume rqrd weight= 1000 then will get weight of the compound required for that normality
Why is Ammonia solution added while preparing EDTA solution?
ammonia solution is added during the preparation of EDTA solution to increase the rate of dissolution of its disodium salt.
How many grams of EDTA do you add to make a 3.7 percent EDTA solution?
To make a 3.7% EDTA solution, you would add 3.7 grams of EDTA to 100 mL of solution.
What is the final concentration of a edta solution if a 1l of 50x stock solution was made using 18.612g of edta mw 372.24?
The molar mass of EDTA is 372.24 g/mol. To calculate the molarity of the stock solution, you need to divide the mass of EDTA used (18.612 g) by its molar mass and then multiply by the dilution factor (50x). Finally, divide this result by the final volume (1 L) to get the final concentration in mol/L.
