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If you mean 100 ml of 1 mM CuSO5.H2O, then dissolve 0.1 mmole CuSO4.H2O in sufficient water to make a final volume of 100 ml.molar mass CuSO4.5H2O = 250 g/mole

250 mg = 1 mmole

25 mg = 0.1 mmol

Dissolve 25 mg in final volume of 100 mls

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