DCPIP is a blue colour when its oxidized and when it is reduced it turns into a colourless solution. DCPIP replaces NADPH the final electron acceptor in the light dependent reaction. So basically, as the reaction continues, the more DCPIP is reduced and the absorbance decreases.
first measure the volume of the sample solution needed to change the blue color of the DCPIP solution into colourless. then, weigh the mass of the sample solution. finally calculate the concentration by using the formula: volume required t change the color of DCPIP solution (dm) per mass of the sample solution (g)
The fullform of DCPIP is 2,6- dichlorophenolindophenol.
Yes, chloroplasts are necessary for the reduction of DCPIP.
DCPIP oxidized is higher in energy
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hmmm.. Safety gloves should be worn in case of a spillage of DCPIP but it's optional. Spilling DCPIP may cause staining on the skin or the surface you are working at. Risks of DCPIP Are extremely low. Do not consume the DCPIP or purposely inhale the substance in case of any dangers or problems related with doing so.
how to prepare .05M glutaraldehyde solution molecular weight is 100.12 how to prepare .05M glutaraldehyde solution molecular weight is 100.12
See the two Related Questions to the left for the answer.The first is how to prepare a solution starting with a solid substance (and dissolving it). The second question is how to prepare a solution by diluting another solution.
Chloroplasts release electrons when stimulated by light the DCPIP accepts the electrons and becomes colourless
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2,6-dichlorophenolindophenol From T.A.
A solution of ammonia can be used to prepare ammoniumsulfate by reacting it with a solution of sulfuric acid to produce a solution of ammonium sulfate, which can be dried if desired to prepare solid ammonium sulfate.
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The concentration of ascorbic acid in an unknown sample can be obtain by conducting a few steps by using the DCPIP test. Firstly, we must obtain the volume (dm ) of the sample solution which are required to turn the DCPIP solution (blue) colour into colourless. After obtaining the volume we use an electronic balance to obtain the mass of the sample solution in gram (g). Then we use the concentration formula of [mass (g) of solute per volume (dm ) of solution] to find out the concentration. Hence. The concentration obtained was the concentration of ascorbic acid.
To prepare a buffer solution which may be acidic. Titrate ethanoic acid (weak acid) with sodium ethanoate(salt).
It can be used to test for vitamin C
it is very easy to prepare working solution from a stock solution we use the formula for this purpose which is: C1V1 = C2V2 C1 is the concentration of the stock solution V1 required volume from the stock solution C2 concentration of the working solution V2 volume of the working solution
smiley by dividing molar solution by 1000
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