0
classic recipes say 0.25% bromphenol blue (0.25g/100ml) in a solution containing a viscous substance like: 40%sucrose, or 15%Ficoll, or 30%glycerol all in water. Personally, I use glycerol.
Wiki User
∙ 16y ago
Wiki User
∙ 14y agowhats the method for preparation of PH solution?
Wiki User
∙ 11y agomercuric chloride 10 gm
bromophenol blue 0.1gm
95% ethyl alcohol 100 ml
mix til dissolve and use .
khalid elfaki
Wiki User
∙ 6y agoDissolve 0,1 g bromophenol blue in 100 mL methanol.
Add your answer:
Earn +20 pts
What is bromophenol blue used for?
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
What is the indicator and color change for a positive test for glucose?
Benedict solution is the chemical indicator for simple sugars.
What is the role of bromophenol blue is plasmid DNA isolation?
Bromophenol blue is the tracking dye in electrophoresis. Being of small molecular size, it races towards the other electrode before the DNA. It is used so that you don't mistakenly let the DNA get washed off the gel and into the buffer solution.
How many gallons of a 12 percent indicator solution must be mixed with a 20 percent indicator solution to get 10 gallons of a 14 percent solution?
10 gallons..
What is the material used to remove an indicator from a neutralized solution how does this work?
it is universal indicator with mixture of alkali
If you add a bromophenol blue solution to .01M HCl what solution color would result after mixing?
yellowish
What does dye contain?
The loading dye comprises bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional in it. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is a pH indicator.
What is bromophenol blue used for?
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
What color is bromophenol blue?
bromothymol blue is an indicator for carbon fioxide, so if it touches carbon dioxide, it turns yellow.but by itself, it is blue.
What happens when bromine reacts with an phenol?
2 bromophenol, 4 bromophenol and hydrogen bromide are formed
What is the indicator and color change for a positive test for glucose?
Benedict solution is the chemical indicator for simple sugars.
What is the role of bromophenol blue is plasmid DNA isolation?
Bromophenol blue is the tracking dye in electrophoresis. Being of small molecular size, it races towards the other electrode before the DNA. It is used so that you don't mistakenly let the DNA get washed off the gel and into the buffer solution.
What indicator has no color at all?
phenolphthalien when added to an acidic solution has a colourless indicator. but shows pink colour for basic solution.
What is the NaCl indicator?
Sodium chloride (NaCl) is not an indicator in analytical chemistry.
How do you prepare 05M glutaraldehyde?
how to prepare .05M glutaraldhyde solution molecular weight is 100.12
When using an indicator is a chemical reaction occurring?
Yes, the indicator react with the solution.
How many gallons of a 12 percent indicator solution must be mixed with a 20 percent indicator solution to get 10 gallons of a 14 percent solution?
10 gallons..
