That depends on what the substances are and how they are combined or mixed. Something like ethano and water can be separated by distillation, the process taking only a few minutes, but something like a mixture of uranium-235 hexafluoride and uranium-238 hexafluoride must be split by centrifuge, the process takinf weeks to separate a small amount. Other ways of separating a mixture include melting (if it is a solid) and using density properties; dissolution, decanting and evaporation (where one or more substances are soluble in the given solute and others are not); recrystallisation (normally to remove impurities).
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