lactose fermenter
No, it doesn't. After performing the Lactose fermentation test, the isolated colonies will turn yellow meaning that the test was negative.
MacConkey agar is yellow (due to the lactose in the agar). When an organism consumes the lactose, the agar turns purple. Purple indicates a positive test. If the organism doesn't use the agar, the agar stays yellow.
yes
ONPG is for the detection of delayed lactose fermentation.
To identify lactose intolerance in children and adults, the hydrogen breath test is used to measure the amount of hydrogen in the breath.
its because Benedict's test only gives a possible test for reducing sugars (all monosaccharides, maltose and lactose).
Theoretically nothing is happend
ONPG test detects only presence of beta galactosidase enzyme whereas lactose fermentation requires the presence of permease as well as beta galactosidase enzyme.
S.sonnei is not a true lactose fermenting bacteria (see.. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=271578&blobtype=pdf)But is a delayed lactose fermenter as it does not have the enzyme permease to transport lactose through its cell wall.However an ONPG lactose fermenting test does not require permease to allow the bacterium to ferment lactose so in an ONPG test the shigella sonnei will appear as lactose fermenting positive.Hope this helps!!2nd year BMS student
Yes. Enterobacter aerogenes ferments lactose and glucose as well as producing gas. It does not produce H2S. Occasionally the slant may revert to an alkaline reaction even though lactose has been fermented. Another lactose test should be formed, such as the reaction on MacConkey agar
Lactose intolerance is diagnosed by a test of the baby's stools (poo) and a blood test. Signs that a baby may be lactose intolerant are the baby's stools are frothy and green in colour, the baby may pull their knees up to their tummy and be irritable and unsettled, or if the baby is breastfed the baby may be constantly on and off the breast but not gaining weight.