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How do you transfer cultured cells from one plate to 6 well plate?
Wiki User
∙ 13y ago
Use a pipette.
Wiki User
∙ 13y ago
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What is the major disadvantage of microbial counts performed by methods other than the serial dilution-agar plate procedure?
The total count includes dead cells as well as living cells.
Does the number of bacteria recorded by the standard plate count method accurately reflect the total bacterial count from the sample?
No. The standard plate count method is an indirect measurement of cell density of only viable bacterial cells. Optical density counting measure entire bacterial sample, the living as well as the dead bacterial cells.
Information storage molecules of cells are called?
Nucleic acids. Deoxyribonucleic acids (DNA) serve as permanent information storage while ribonucleic acids (RNA) serve as information transfer media as well as other functions..
Why do muscle cells and a brain cells need a lot of energy?
Well, muscle cells are used to do work. And brain cells are used constantly.
What is the diference between plant and animal cells?
Well, the main two are: 1. A cell wall and 2. Chloroplast Plant cells have cell walls and chloroplasts and animal cells do not. Animal cells have centrioles and plant cells do not.
Why kasumi-1 cells transfected with a plasmid and selected in drug for 21 days died after there multiplycation in a month?
AML cells in general don't transfect very well & typically die shortly after. My guess is that you cultured ghost-cells for 21 days.
Do plants reproduce sexually or asexully?
well there are the male cells of the plant and female parts. bees transfer......
What are some advantages and disadvantages of the serial dilution agar plate technique?
the total count includes dead as well as living cells
What is the major disadvantage of microbial counts performed by methods other than the serial dilution-agar plate procedure?
The total count includes dead cells as well as living cells.
What is meant by the term cultured?
To call a person cultured is to infer that the person has refined taste, manners and is well educated. Another meaning is used in Biology, when cells grown or propagated in an artificial medium are said to be "cultured".
What are synonyms for cultured?
refined, intellectual, educated, sophisticated, enlightened, well-informed, urbane, highbrow
How are plant cells and animals cells similar?
well they are both eukaryotic so they are very similar. However animal cells do not have a chloroplasts, while plant cells do. Also, animal cells have centrioles while plant cells do not. Another difference is that during mitosis and meiosis plant cells produce a cell plate, while animal cells produce cleavage furrow.
Why do you use an inoculating loop to transfer fromt he culture plates to the culture tubes?
You can transfer more because of the adhesive interactions of the water create that drop in the loop and you can spread it more easily. Also a needle could pierce the gel you transfer to because of its pointy end. The loop on the other hand distributes its force over a greater area so it does not damage the gel. This is a good answer if going from the tube to the plate, but backwards for the actual question. You use a loop to pick up a well isolated colony from your streak plate to introduce into the culture. You also can use a needle, but you won't get as many cells. However, in my work I just use sterilized toothpicks.
What is doubling time for HepG2?
48 h (DMEM containing 10% FBS, 5x104 cells/well in a 24-well plate); Wu Wen-Sheng, Oncogene (2003) 22, 955-963
Does the nuclear transfer process used in cloning kill cells?
Well ... it kills the cell(s) you took the nucleus out of, (you've got to clean out two) But you kill more cells whenever you scratch you head. (by a thousand times)
Who are sophisticated people?
They are those of the upper class and are well educated and cultured. They are well mannered and smart. They have a wide knowledge of the world and lack naivete and simplicity.
What is the antonym of cretin?
Sophisticate, Well-cultured individual, any descriptive noun involving intelligence or cultural proficiency.
