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How many cells should be seeded in a 6-well plate for optimal growth and experimental results?
For optimal growth and experimental results in a 6-well plate, it is recommended to seed around 1-2 million cells per well.
What are the available cell culture plate sizes for conducting experiments in a laboratory setting?
Common cell culture plate sizes used in laboratory settings include 6-well, 12-well, 24-well, 48-well, and 96-well plates. These plates provide different numbers of wells or compartments for growing cells and conducting experiments. Researchers choose the appropriate plate size based on the specific needs of their experiments.
What is the volume per well in a 384 well plate?
The volume per well in a 384 well plate is typically around 50-100 microliters.
How much volume can a 96-well plate hold?
A 96-well plate can hold a total volume of around 300-400 microliters per well.
Cells convert the energy from gluecose into?
Cells convert the energy from glucose into ATP (adenosine triphosphate) through a series of chemical reactions in a process called cellular respiration. ATP is the primary molecule used by cells to store and transfer energy for various cellular functions.
How many cells should be seeded in a 6-well plate for optimal growth and experimental results?
For optimal growth and experimental results in a 6-well plate, it is recommended to seed around 1-2 million cells per well.
Why kasumi-1 cells transfected with a plasmid and selected in drug for 21 days died after there multiplycation in a month?
AML cells in general don't transfect very well & typically die shortly after. My guess is that you cultured ghost-cells for 21 days.
Do plants reproduce sexually or asexully?
well there are the male cells of the plant and female parts. bees transfer......
What are some advantages and disadvantages of the serial dilution agar plate technique?
the total count includes dead as well as living cells
What are synonyms for cultured?
refined, intellectual, educated, sophisticated, enlightened, well-informed, urbane, highbrow
What is meant by the term cultured?
To call a person cultured is to infer that the person has refined taste, manners and is well educated. Another meaning is used in Biology, when cells grown or propagated in an artificial medium are said to be "cultured".
What are the available cell culture plate sizes for conducting experiments in a laboratory setting?
Common cell culture plate sizes used in laboratory settings include 6-well, 12-well, 24-well, 48-well, and 96-well plates. These plates provide different numbers of wells or compartments for growing cells and conducting experiments. Researchers choose the appropriate plate size based on the specific needs of their experiments.
Who are sophisticated people?
They are those of the upper class and are well educated and cultured. They are well mannered and smart. They have a wide knowledge of the world and lack naivete and simplicity.
Why do you use an inoculating loop to transfer fromt he culture plates to the culture tubes?
You can transfer more because of the adhesive interactions of the water create that drop in the loop and you can spread it more easily. Also a needle could pierce the gel you transfer to because of its pointy end. The loop on the other hand distributes its force over a greater area so it does not damage the gel. This is a good answer if going from the tube to the plate, but backwards for the actual question. You use a loop to pick up a well isolated colony from your streak plate to introduce into the culture. You also can use a needle, but you won't get as many cells. However, in my work I just use sterilized toothpicks.
What is the antonym of cretin?
Sophisticate, Well-cultured individual, any descriptive noun involving intelligence or cultural proficiency.
What is doubling time for HepG2?
48 h (DMEM containing 10% FBS, 5x104 cells/well in a 24-well plate); Wu Wen-Sheng, Oncogene (2003) 22, 955-963
Does the nuclear transfer process used in cloning kill cells?
Well ... it kills the cell(s) you took the nucleus out of, (you've got to clean out two) But you kill more cells whenever you scratch you head. (by a thousand times)
