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Why kasumi-1 cells transfected with a plasmid and selected in drug for 21 days died after there multiplycation in a month?
Ajorer
AML cells in general don't transfect very well & typically die shortly after. My guess is that you cultured ghost-cells for 21 days.
Wiki User
∙ 14y ago
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How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
When inserting a new gene into plasmid why is it important that the same restriction enzyme is used on both the DNA selected and the plasmid?
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When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
What is the function of the Ti plasmid?
what is the function of the plasmid
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
Analogy for plasmid?
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
What is a self transmissible plasmid?
A plasmid which encodes genes for its own transfer.
What is a self-transmissible plasmid?
A plasmid which encodes genes for its own transfer.
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication.
What type of plasmid is the source of the problem with antibiotic resistance?
R plasmid.
What occurs first in the production of a recombinant plasmid?
Isolation of a plasmid from a bacterium
