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You can determine if your bacteria contain a plasmid by performing a plasmid extraction followed by gel electrophoresis to visualize the presence of plasmid DNA. Other methods include PCR amplification of plasmid-specific sequences or using molecular Biology techniques like restriction enzyme digestion to confirm the presence of a plasmid.
What else can I help you with?
Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow where?
The bacteria containing the plasmid with the integrated eukaryotic gene would grow in a selective medium that supports the growth of bacteria carrying the plasmid. This medium would typically contain an antibiotic or a specific nutrient that selects for bacteria with the plasmid.
How can bacteria be transformed with recombinant plasmid?
Bacteria can be transformed with recombinant plasmid by introducing the plasmid into the bacterial cell through a process called transformation. This allows the bacteria to take up the recombinant DNA from the plasmid and express the desired gene or trait encoded in the DNA.
What is the small ring of DNA in bacteria?
Bacteria generally have one (and only one) circular chromosome. However, they can also contain smaller rings of DNA called plasmids. These plasmids are generally not essential to cellular functions, but serve a variety of purposes. For example, they can serve as cloning vectors.
The takes up the plasmid. It now contains the human gene?
I think I know the answer... it's 5
How can one effectively clone a gene into a plasmid?
To effectively clone a gene into a plasmid, the gene of interest and the plasmid are cut with the same restriction enzymes to create compatible ends. The gene is then inserted into the plasmid using DNA ligase to seal the ends. The plasmid is then introduced into a host cell, such as bacteria, where it can replicate and express the cloned gene.
Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow where?
The bacteria containing the plasmid with the integrated eukaryotic gene would grow in a selective medium that supports the growth of bacteria carrying the plasmid. This medium would typically contain an antibiotic or a specific nutrient that selects for bacteria with the plasmid.
What organisms have plasmid?
Bacteria
What is col plasmid?
A plasmid (free bit of DNA) in bacteria that produces colicin to kill other strains of bacteria.
Is human cell contain plasmid?
Plasmid is the property of prokaryotic cell i.e. of bacteria generally no mamalian cell have plasmid but ya there can be chances to have lillte part of DNA sequence to be simillar that of plasmid DNA sequence in prokaryotes
Why the cell do not contain plasmid if they not grow in antibiotics?
If you do not give antibiotics, the plasmid may lost after a few generation. it is because bacteria thinks that the extra DNA (plasmid) is not needed as the antibiotic is not present. That is the reason for keeping the culture always in antibiotic selection pressure.
Why does bacteria that contains plasmid glow in uv light?
the plasmid contains a certain gene, which codes for the "Green Flourescent Protein." So you put the plasmid in the bacteria, the plasmid starts making that protein in the bacteria, and boom you've got glowing bacteria. works for bunnies and monkeys too, apparently =)
How can bacteria be transformed with recombinant plasmid?
Bacteria can be transformed with recombinant plasmid by introducing the plasmid into the bacterial cell through a process called transformation. This allows the bacteria to take up the recombinant DNA from the plasmid and express the desired gene or trait encoded in the DNA.
How is it possible to splice foreign DNA into a plasmid without altering the bacteria's ability to function normally?
A plasmid is an extra-chromosomal element. Strictly speaking, it is not a part of the bacterial mail genome. Plasmids, when first discovered in bacteria, were observed to contain genes that confer special features to the bacteria (like drug resistance). Also, a plasmid is capable of autonomous replication, which means it has its own origin of replication and does not have to wait for the bacterial genome to be replicated. When a foreign gene is inserted into a plasmid, the plasmid has to be transformed into the bacteria. Once transformed, the cDNA in the plasmid is able to replicate every time the plasmid replicated. This does not have any affect on the normal functioning of the bacteria. The organism is merely being used to make copes of the foreign gene and/or harvest an important protein product.
Does a bacteria cell have a plasmid?
Yes
Which organelles inside bacteria make green fluorescent protein?
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.
What is the small ring of DNA in bacteria?
Bacteria generally have one (and only one) circular chromosome. However, they can also contain smaller rings of DNA called plasmids. These plasmids are generally not essential to cellular functions, but serve a variety of purposes. For example, they can serve as cloning vectors.
What is the function of a plasmid?
Plasmids have many different functions. They may contain genes that enhance the survival of an organism, either by killing other organisms or by defending the host cell by producing toxins. Some plasmids facilitate the process of replication in bacteria.
