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How can bacteria be transformed with recombinant plasmid?
Bacteria can be transformed with recombinant plasmid by introducing the plasmid into the bacterial cell through a process called transformation. This allows the bacteria to take up the recombinant DNA from the plasmid and express the desired gene or trait encoded in the DNA.
What is curing of plasmid?
Bacteria possess extra chromosomal DNA,called plasmids. Often it carries functional genes for the resistance of bacteria (example: Aromotic compound degrading genes). Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin or acridine orange!
Is human cell contain plasmid?
Plasmid is the property of prokaryotic cell i.e. of bacteria generally no mamalian cell have plasmid but ya there can be chances to have lillte part of DNA sequence to be simillar that of plasmid DNA sequence in prokaryotes
How does plasmid allow for antibiotic resistance?
A plasmid (conjugative plasmid) that has a resistance gene on it, can transfer itself to another bacterial cell (called conjugation) or assist in the transfer of a non-conjugative plasmid that has a resistance gene to another cell (called mobilization). Whichever way it happens, once the plasmid is transfered to the new cell, this cell too may show signs of resistance to the particular antibiotic. This is one of the ways of the speard of resistance amongst bacteria
What organisms have plasmid?
Bacteria
What is col plasmid?
A plasmid (free bit of DNA) in bacteria that produces colicin to kill other strains of bacteria.
What is the function of a plasmid?
Plasmids have many different functions. They may contain genes that enhance the survival of an organism, either by killing other organisms or by defending the host cell by producing toxins. Some plasmids facilitate the process of replication in bacteria.
Why does bacteria that contains plasmid glow in uv light?
the plasmid contains a certain gene, which codes for the "Green Flourescent Protein." So you put the plasmid in the bacteria, the plasmid starts making that protein in the bacteria, and boom you've got glowing bacteria. works for bunnies and monkeys too, apparently =)
What is a multicopy plasmid?
Every plasmid has a copy number that reflects the average number of copies of a certain plasmid inside a host cell(usually a bacterial cell). So a multicopy plasmid, exist in multiple copies in any given bacteria. It is believed that the higher the copy number is, the more efficient the plasmid is at replicating itself.
How can one effectively clone a gene into a plasmid?
To effectively clone a gene into a plasmid, the gene of interest and the plasmid are cut with the same restriction enzymes to create compatible ends. The gene is then inserted into the plasmid using DNA ligase to seal the ends. The plasmid is then introduced into a host cell, such as bacteria, where it can replicate and express the cloned gene.
What two mating bacteria ame cell-to-cell contact by a thin temporary cytoplasmic bridge through which the plasmid from the cell travels to the other cell?
The two mating bacteria are called donor and recipient cells. The temporary cytoplasmic bridge connecting them is known as a pilus. During conjugation, the plasmid carrying genetic material passes from the donor cell to the recipient cell through this pilus.
Why the cell do not contain plasmid if they not grow in antibiotics?
If you do not give antibiotics, the plasmid may lost after a few generation. it is because bacteria thinks that the extra DNA (plasmid) is not needed as the antibiotic is not present. That is the reason for keeping the culture always in antibiotic selection pressure.
