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How can foreign pieces of DNA be transfered into organisms?
By a vector such as a plasmid or by a BAC or YAC.
What is a helper plasmid?
A helper plasmid is one that allows for the beginning of replication and transfer of other plasmids from a donor to a recipient. Without a helper plasmid, transposons will not be expressed in the recipient.
What is the term for a plasmid that contains a foreign gene?
Recombiant DNA
What occurs first in the production of a recombinant plasmid?
In the production of a recombinant plasmid, the DNA of interest (insert) and the plasmid vector are both cut with restriction enzymes to create compatible ends. These cut fragments are then ligated together using DNA ligase to produce the recombinant plasmid.
When is a plasmid considered a recombinant plasmid?
A plasmid is considered recombinant when it contains DNA sequences from two different sources that have been artificially combined, often through genetic engineering techniques like restriction enzyme digestion and ligation. This results in a plasmid with modified or additional genetic material compared to its original form.
How can foreign pieces of DNA be transfered into organisms?
By a vector such as a plasmid or by a BAC or YAC.
How are plasmid classification on their property?
Plasmids are classified as: 1. F plasmid 2. R plasmid 3. Col plasmid F plasmids for fertility factor, it transfers its plasmid to the non fertile making it fertile. R plasmid for certain antibiotic resisitivity..for eg, ampicillin resistance. Col are certain proteins which when produced doesnt let other organisms to invade its cell.
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
What are plamids?
A plasmid is a circular ring of DNA that is separate from the main 'cluster' of DNA in a bacterium. They are not usually found in any other organisms apart from bacteria. They are used in genetic modification where and extra gene is inserted into the plasmid ring so the bacteria produces substances (such as insulin).
How can i know if my bacteria contain plasmid or not?
You can determine if your bacteria contain a plasmid by performing a plasmid extraction followed by gel electrophoresis to visualize the presence of plasmid DNA. Other methods include PCR amplification of plasmid-specific sequences or using molecular biology techniques like restriction enzyme digestion to confirm the presence of a plasmid.
What is a self transmissible plasmid?
A plasmid which encodes genes for its own transfer.
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication.
What is a helper plasmid?
A helper plasmid is one that allows for the beginning of replication and transfer of other plasmids from a donor to a recipient. Without a helper plasmid, transposons will not be expressed in the recipient.
How many plasmid slots can you have in BioShock 2?
You can have a maximum of 8 plasmid slots.
What is the term for a plasmid that contains a foreign gene?
Recombiant DNA
Is the plasmid found in prokaryotic or eukaryotic cells?
The plasmid is found in prokaryotic cells.
