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Anylase works best in a slightly basic pH so a very acidic pH will denaturate the enzyme making it unable to do it's job.

Effects of pH

Enzymes are affected by changes in pH. The most favorable pH value - the point where the enzyme is most active - is known as the optimum pH.

Extremely high or low pH values generally result in complete loss of activity for most enzymes. pH is also a factor in the stability of enzymes. As with activity, for each enzyme there is also a region of pH optimal stability.

In addition to temperature and pH there are other factors, such as ionic strength, which can affect the enzymatic reaction. Each of these physical and chemical parameters must be considered and optimized in order for an enzymatic reaction to be accurate and reproducible.

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13y ago
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16y ago

Yes. Amylase is an enzyme that breaks starch (a complex carbohydrate) into its simple sugar, glucose. Starch is also known as amylose. Enzyme activity is sensitive to the temperature and pH of its environment. At the wrong temperature or pH, the enzyme's structure will degrade. When this happens the active site of the enzyme changes shape and is no longer able to interact with its substrate. Here the substrate for amylase is amylose. When an enzyme looses its shape, it is described as being denatured.

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Q: How does temperature and pH affect amylase activity and why?
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Related questions

State one other factor apart from temperature that will affect the rate of reaction of amylase?

pH and temperature


What factors affect the activity of enzymes?

Enzymes work best in the pH and temperature that they are " designed " for. A pepsin enzyme works best in the low pH environment of the stomach, while amylase works best at mouth temperature and ~ 7 pH. Heat and out of range pH can denature enzymes and not only affect their activity but inactivate them.


What is the opitmal pH for salivary amylase?

identify two pH values at which salivary amylase activity stop?


At what pH did you see the highest activity of salivary amylase?

pH of about 7


What may affect enzyme activity?

Temperature, pH, salt concentration


Does amylase activity increase or decrease in the stomach?

The activity of amylase decreases drastcially due to the very low pH this is one of the reasons more amylase is added by the pacreas.


Temperature and can affect enzyme activity?

Ph level, enzyme concentration, substrate concentration etc


What 2 things can affect enzyme activity?

Temperature.Ph Levels.Specificity.Concentration of enzymes and substrate.Cofactors/co enzymes.Inhibitors.


What are the best conditions in pH for amylase protese and lipase?

Amylase has an optimal pH range of 6.7 - 7.0 and an optimal temperature of around 37 degrees Celsius or 98.6 degrees Fahrenheit. This is what would be expected of an enzyme that needs to be in its most active form in the saliva of the mouth.


What conditions affects the function of enzymes?

The activity of an enzyme is affected by temperature, pH and the concentration of the substrate.


What is the optimum pH for amylase activity at pH?

The optimum pH of a bacterial amylase depends on the species of bacterium. One review of different species found optima ranging from pH 4 to 11.To take one example, the α-amylase from Bacillus amyloliquefaciens (BAA) has an optimum pH of 6.Bacterial amylases are important in a number of industrial processes.


9th grade level biology question. Temperature and what can affect enzyme activity?

effect of ph and concentration of subtrate