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For checking the clones quick screening of the colonies helps. the colonies grown the selective medium should be patched on the respective medium and allowed to grow in 37degree incubator. the next day the grown colonies should be inoculated in a liquid specific medium and incubated overnight in a shaker. the very next day the cells are pelleted out and BPB and phenol chloroform of 40microliters,20microliters resp.,to a 1.5microliters culture. quick vortexing should be done followed by spin at 12K for 20mins. later the supernatant is separated and loaded on gel.

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