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Basically, transformation refers to the uptake and incorporation of naked genetic material by a competent cell. Most bacterial cells are not naturally competent(able to take up naked genetic material). The kinds that are use a type 4 pilli to get the genetic material into the cell. This process is very rare in nature, since it requires naked genetic material to be present, from a lysed cell, as well as a competent cell to take up the genetic material. When the DNA/RNA is taken up, it is often degraded by restriction enzymes. If it is not degraded it can exist as extra chromosomal DNA, much like a plasmid, or it can be incorporated into the cells chromosome. Scientists use heat, pH, or osmotic shock in lab to make cells artificially competent.

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