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What else can I help you with?
Why are the ptm important?
Post-translational modifications (PTMs) play a crucial role in regulating protein activity, stability, localization, and interaction with other molecules. They can affect the function and properties of proteins, influencing various cellular processes such as signaling pathways, gene expression, and cell cycle regulation. Understanding PTMs is essential for deciphering the complexity of cellular processes and disease mechanisms.
How can we take advantage of peptide de novo sequencing to analyze the biological function of the peptideprotein?
Complete characterization of peptides/proteins, including post-translational modifications (PTMs), sequence mutations and variants, is very challenging. There are two approaches to determine the sequence of peptide/protein by mass spectrometry: database search and de novo sequencing. Database search approach compares acquired mass spectra to a database of known protein sequences to identify the protein sequences.
Why the bacteria don't have post translational modifications?
They in fact do. Check out a review "Postranslational Protein Modification in Archaea" by Jerry Eichler. The problem is that took a long time to discover this so it was assumed that they don't have any PTMs but this is wrong.
