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Q: How restriction enzyme recognize a DNA sequence while poshphate back bone is present out side the DNA seqence?
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Related Questions

Process essays present the process in?

sequence


Why don't bacterial chromosomes get cut out with the restriction enzymes present in the cell?

Methyl groups are attached to all bacterial genetic material to protect it from its own restriction enzymes.


How do you recognize a biome?

You can recognize a biome based on the features or characteristics present. Weather and vegetation are excellent features of a specific biome.


A process essay should present what in sequence?

The process being explained


Past tense for recognize?

present: recognise (British English), recognize (American English)past: recognised (British English), recognized(American English)


Is thymine present in any Dna sequence?

Yes, along with A, G and C, it is present in any and all Dna sequences.


How old do you have to be to ride on back of a motorcycle in California?

At the present time there is no age restriction. However a safety helmet is required.


How are plasmids and restriction enzymes used in genetic engineering?

Plasmids, circular double stranded DNA molecules, are used as a shuttle to transport a gene of interest into a foreign host cell for a number of reasons. These reasons include either maintenance of the plasmid within the cytoplasm, or integration of the foreign DNA into the nuclear DNA of the host cell. If the plasmid is maintained in the cytoplasm this often to express a heterologous protein, and has significant application in the commercial sector where bioreactors generate substantial income from production of proteins/enzymes for a multitude of applications. Thus an organism has been genetically engineered with a specialized protein expression vector (plasmid) to express a foreign protein. Expression of foreign proteins are also essential for protein studies where a purified form of a protein is required to assess and characterize its structure and function. Another application of plasmid transfer to the cytoplasm of a bacterial cell is present in increasing the copy number (amount of plasmids) of the DNA. When large numbers of plasmids have been generated the cells can be lysed and the plasmids isolated for further use and additional transformation experiments. Construction of DNA libraries also require the use of plasmids to have genomic DNA of the organism in question inserted within them and maintained for screening of genes of interest. Some unique vectors based on the principles of Ti-plasmid of Agrobacterium species can transfer its DNA into the genome of the host cell by a process of non-homologous end joining that is not sequence specific. This insertion does tend to be localized to certain areas of the genome, and is a popular transformation technique of dicot plant species. Getting back to restriction enzymes, and straining their importance as genetic engineering would not be possible without their existence. The Restriction enzymes can be compared to molecular scissors that excise DNA sequences from a larger DNA segment. They are naturally occurring enzymes present in all living organisms and a large number have been made commercially available for industrial or research purposes. The restriction enzyme recognizes a specific sequence within a DNA sequence known as a restriction site, and can make a single stranded or double stranded cut depending on the restriction enzyme used. The cut if double stranded generates two possible scenarios, either a blunt end or a sticky-end overhang. Different restriction enzymes recognize different restriction sites and provide an opportunity to isolate specific regions of DNA when sequence information is available. After isolation of a specific DNA sequence, primers are designed, and the sequence is amplified by means of PCR. In parallel a mixture is prepared containing a plasmid with a multiple cloning site containing numerous restriction sites. The plasmid is then incubated with one or 2 restriction enzymes depending on the sequence of the DNA to be inserted, and allows for orientation of the gene to ensure expression under a valid promoter in the plasmid. Natural base pairing of DNA takes place and complementary sticky ends are ligated with DNA ligase to incorporate the gene of interest into the plasmid.


What is the correct sequence of historical developments leading to your present knowledge of cells?

Put yo


What is sequence in a story?

Sequence is a line of events in chronological order, usually starting with the event farthest back in time and leading up to the present, or end, of the book.


How is the biuret test helpful?

because it helps to recognize any protein present within food


Is neha choudhary the present miss India?

I don't recognize the name at all but I will guess and say "NO"


Occurs when the sequence of events in a work is interrupted to present information from a previous time?

Flash Back


Paragraph relates a sequence of events or tells a story?

Indeed, yes, a paragraph relates a sequence of events or tells a story. It can also be informative, present arguments, or compare-contrast.


Is losing an adverb?

No. It can be a verb form (present participle) or an adjective. Few dictionaries recognize the adverb form losingly.


What is Gap Closure methodology?

Its a technique adopted during DNA sequencing in which while joining the contigs we find whether there is any sequence present in between the contigs or not.If there is a sequence inbetween they re sequence the ends of the DNA.If not PCR is used to close the gaps.


What is the difference between sequence and series?

A series is a set of numbers to work with, which may vary as the problem changes. A sequence is a set of numbers which must be in the same order and they do not vary. The sequence may not be in a "logical" order such as 123. They may be 132 or 213, but they always present in the same order. The series can be any numbers but they may present out of "order". These numbers can present without some of the "logical" numbers being present such as 124. If the problem changes, the numbers might in the series. If the problem changes and the "2" is no longer needed, the series can change to 134. In this case, the series changes to fit the problem...the sequence is always in the same order and uses the same number.


Is follows an adverb?

No. The word follows is the third person singular, present tense of "to follow" (comes after in sequence, or trails).


What food do christians eat?

Most present day Christians have no food restriction beliefs, so they eat the same food as every one else in their area.


What are gustatory receptors?

Gustatory receptors are also present in the mouths of animals. These are novel proteins which are able to recognize a variety of chemicals.


Can anybody go to the callisto?

At present there have been no manned missions beyond the Moon, so although there are no restriction (eg visa requirements) for going to Callisto, it would not be easy.


Which is the correct sequence of historical developments leading to your present knowledge of cells?

compound light microscope - cell theory - electron microscope


Which protein is present in spider net?

Spider Web Glue: Two Proteins Expressed from Opposite Strands of the Same DNA Sequence


What type of math did Fibonacci do?

Fibonacci found a way to present mathematical numbers so that each number in the sequence is the sum of the two previous numbers. For example, if the sequence starts at 0 and 1, then next number in the sequence is 1, the next number would be 2, and then the next number would be 3, and then 5.


Why you add distilled water in restriction digestion of lambda DNA along with enzyme and assay buffer?

We generally add milliQ (DNase free) water. For digestion water is added usually. It provides right Hydrogen bonds between enzyme amino acid residues and bases present in the restriction sites. Utpal Roy