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How the protein sample without aromatic ring containing aminoacids can be observed under spectrophotometer and at what wave length?
Wiki User
∙ 12y ago
A general protein assay for determining protein concentrations (of proteins with no aromatic amino acids) would be using the Bradford Method. The binding of this Coomassie Brilliant Blue G-250 dye to proteins will cause a shift in the dye's maximum absorbance wavelength. The free dye is red and absorbs at 465nm, but the blue protein-bound dye can be visualized at 595nm.
You will need to construct a standard curve of BSA, for example. Then use the trend line from that curve to determine your protein concentration.
Reference: Bradford, M. 1976, Analytical Biochemistry, vol. 72, p. 248.
Wiki User
∙ 12y ago
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