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How would you define pure culture sterile and contamination in the isolation and purification of bacteria?
Wiki User
∙ 10y ago
A pure culture is defined as a population containing only a single species or strain of bacteria. Contamination means that more than one species is present in a culture that is supposed to be pure.
Wiki User
∙ 10y ago
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How would you define pure culture sterile culture and contamination in the culture and isolation of bacteria?
A pure culture contains only one bacteria and no other. A sterile culture has only one bacteria in it and is usually used for transport. Contamination is something that doesn't belong. It can even be a small bit of plastic or wire. It is the opposite of a pure culture. The whole idea when working with isolating bacteria is to figure out which bacteria (or microbe) is causing an infection or disease in a patient. Your job is to help to figure this out so that the proper treatment can be started as soon as possible.
Which culture medium preparation is most commonly utilized in isolation of pure culture?
Solid media is used for the isolation of bacteria as pure culture. 'Agar' is most commonly used to prepare solid media.
Why are antibiotics frequently added to sabouraud agar for isolation of fungi from clinical samples?
So bacteria do not contaminate the fungi culture.
Why is it to obtain to maintain a pure culture?
control the contamination
What bacteria are more sensitive to the culture process?
There is no one answer to this question as different bacteria have different sensitivity to the culture process. However, some bacteria are generally more sensitive to the culture process including those that are fastidious (require specific nutrients or growth conditions), those with delicate cell membranes, and those that are susceptible to contamination by other microorganisms. Examples of bacteria that may be more sensitive to the culture process include species of Mycobacterium and Neisseria.
How would you define pure culture and sterile and contamination in the isolation and purification of bacteria?
A pure culture is defined as a population containing only a single species or strain of bacteria. Contamination means that more than one species is present in a culture that is supposed to be pure.
How would you define pure culture sterile culture and contamination in the culture and isolation of bacteria?
A pure culture contains only one bacteria and no other. A sterile culture has only one bacteria in it and is usually used for transport. Contamination is something that doesn't belong. It can even be a small bit of plastic or wire. It is the opposite of a pure culture. The whole idea when working with isolating bacteria is to figure out which bacteria (or microbe) is causing an infection or disease in a patient. Your job is to help to figure this out so that the proper treatment can be started as soon as possible.
What are the abnormal results of a sputum culture?
The presence of bacteria and white blood cells on the Gram stain and the isolation of a microorganism from culture, other than normal flora contamination, is evidence of a lower respiratory tract infection.
Who is responsible for developing the culture plate method for isolation of bacteria?
Robert Koch
Which culture medium preparation is most commonly utilized in isolation of pure culture?
Solid media is used for the isolation of bacteria as pure culture. 'Agar' is most commonly used to prepare solid media.
Why are antibiotics frequently added to sabouraud agar for isolation of fungi from clinical samples?
So bacteria do not contaminate the fungi culture.
What are the prevention pure culture contamination?
if contamination is noted in a subculture sample, what should be to obtain a pure culture
What is cross contamination in animal tissue culture?
Cross contamination means the growth of microrganism in animal cell culture.
How does rifampicin act in the selection of agrobacterium?
Rifampicin resistance is encoded by a gene residing on the chromosome of Agrobacterium, so its purpose is to prevent contamination by other bacteria when it is grown in culture
Why is the production of an isolation streak key to the identifcation of a bacterium grown in culture?
Isolation streaking was first introduced by Robert Koch as an improvement on the dilution method of isolation of bacterial cultures. Isolation is achieved by taking a minute sample from within one bacterial colony and putting it in a sterile medium so that only that organism will grow. The assumption here is that a bacterial colony has ways of ensuring that no other bacteria will grow within its colonies, primarily by the use of antibiotic compounds that it produces and secondarily by the fact that it will consume available medium before any other organisms can establish themselves. Contamination of a sample by bacteria other than the one desired can lead to improper identification and poor test results. Good isolation such as that provided by the isolation streak method addresses this concern, is cost-effective, and produces acceptable results.
How can man culture and society be studied in isolation?
how culture observed?
Why is it to obtain to maintain a pure culture?
control the contamination
