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The best way is to filter sterilize the solution using a nylon membrane/ syringe filter, of 0.45 micron pore size. If needed, dissolve Urea in autoclaved water and then filter sterilize.

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Tiara Lebsack

Lvl 13
3y ago

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How would you sterilize a urea media that is heat sensitive?

One way to sterilize a heat-sensitive urea media is by using filtration methods, such as membrane filtration or sterile filtration. This involves passing the media through a sterile filter that can remove microorganisms while preserving the integrity of the media. Another option is to use chemical sterilization methods, such as ethylene oxide gas sterilization or gamma radiation, ensuring that the sterilization process does not adversely affect the composition and quality of the media.


What control methods will sterilize but not break down heat sensitive material?

Some control methods that can sterilize heat sensitive materials without breaking them down include ethylene oxide (ETO) gas sterilization, hydrogen peroxide gas plasma sterilization, and liquid chemical sterilants like peracetic acid. These methods can effectively sterilize materials without exposing them to high heat that could cause damage.


Which media are not autoclavable?

Some examples of media that are not autoclavable include heat-sensitive materials like plastics, certain chemicals, and some types of agar that can be altered or degraded by the high temperatures and pressure of the autoclave. It's important to carefully check the compatibility of each specific type of media with autoclaving before attempting to sterilize it in this way.


How would you sterilize a heat-sensitive growth medium containing thermoduric bacteria?

One option is to use chemical sterilization methods such as filtration using a sterilizing-grade filter or using ethylene oxide gas. These methods can effectively sterilize without subjecting the heat-sensitive growth medium to high temperatures that may compromise its quality or integrity. It is important to ensure proper ventilation and follow safety protocols when using chemical sterilization methods.


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