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How you do widal test?

Updated: 9/19/2023
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12y ago

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Widal test is done by qualitative method or quantitative method.

Quantitative method:-Take a set of 8dry clean test tubes.

Place 1.9ml saline in tube no.1 and 1ml saline in rest of tubes. Add 0.1ml serum in 1st tube mix well it is dilution of 1:20

Transfer 1ml diluted serum from tube no.1 to tube no. 2 and so on.

Through tube no.7 discard 1ml of fluid after mixing.

leave 1ml saline in tube no.8.

Add one drop of appropriate antigen(use only that antigen suspension which has given a positive reaction in screening test.

Mix well and incubate overnight at 37.c

Examine for agglutination.

Interpretation:- Only a titre above 1:80 should be considered as significant

A rising titre (done each week)is considered to be definite evidence of infection.

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12y ago
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Related questions

What is false positive widal test?

What is false positive in widal test


What is the difference between widal slide test Antigen and widal tube test antigen?

widal slide is easier and it save time but widal tube test is not easy and it takes time.


Will Widal Test after positive resul after treatment in Typhoid?

I mean widal test result positive after treatment


How do you read widal test?

Isolated Widal test reading does not have much clinical significance. Rising titre of Widal test done one week apart, in a febrile patient, indicates typhoid fever. You can have such rising titre of Widal test in healthy patient, after dose of typhoid vaccine.


What significant titer for widal's test?

1:80 titre is significant for determination of widal positive.


Name of the blood test for typhoid?

WIDAL


What are the diagnosis for typhoid fever?

widal test


Do antibiotics affect widal test results?

To a certain extent yes.The post antibiotics Widal Test will have Low titers of agglutinins.Hence the test may be reported as False Negative.


How you can confirm that typhoid is eradicated with treatment?

widal test.


What is a manual method?

manual test of widal method


Why widal test appears negative in first week of typhoid fever?

In Widal test, you measure the antibodies. It takes minimum one week to develop the antibodies of typhoid bacteria.


How would you distinguish between an incubatory carrier and a chronic carrier of typhoid fever?

Widal test is negative in incubatory carrier. Widal test is always positive in chronic carrier.