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If you ran your DNA samples on a 0.8 agarose gel would you get the same results if you ran your samples on a higher percentage agarose gel?
Wiki User
∙ 15y ago
Yes and no - it will not change the result, as such, but the resolution will be affected. The higher the density (percentage) of agarose the more it will retard your DNA sample, so larger DNA fragements run more slowly and at high percentage won't run into the gel properly. Essentially, high percentage (2%) gels are ideal for looking at small DNA fragments (100bp) and low percentage (0.7%) are for large DNA fragments (2kb). I find this webpage from Fermentas very useful for deciding what percentage to use and has lots of other useful bits on it:
http://www.fermentas.com/techinfo/appendix/appendixtables1.htm#DNAMigration
Wiki User
∙ 15y ago
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Why was it important for mendel study such a large sample of pea plants?
(Apex Learning) A higher sample size gives more accurate results.
What is the relationship between the size of the DNA fragment and the distance that it migrates from the well?
. Because DNA is a negatively charged molecule, it will migrate through the gel toward the positive electrode (recall that opposite charges attract). The rate of migration of DNA through the agarose depends on the size of the DNA fragment. The smaller the fragment, the faster it can move through the gel. Another important factor is the concentration of agarose in the gel. The higher the concentration of agarose, the more it slows down the movement of all the DNA fragments.
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The percentage of water in a baby is around 78 to 84 percent. This is higher than in an adult body which has 57 to 60 percent.
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The percentage of the net movement of water into a cell through the process of osmosis is that the outside would be higher than the water on the inside of the cell. For example, there would be 95 percent of water on the outside, which is a higher concentration, and the inside would be 90 percent.
Island a has an area of 30 square kilometers island b has an area of 400 square kilometers the islands are near each otherwhich is the hiest percentage?
Island a has less biodiversity and higher percentage of edge effects than island b.
Why you have the range of concentration of agarose in gel electrophoresis?
increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
Why are controls necessary when performing biochemical test?
Control samples are samples that someone has specifically created to have know concentrations of the compound(s) of interest. This includes blank samples with zero concentration. The Controls are mixed in with the real samples so the analyst does not know which are which. The analyses are conducted by standard methods and the results reviewed. If the work is done properly the values for the controls will be the same or within an expected range of variance as the known concentrations. The values of the real samples are then likely to be correct. This is a quality control or quality assurance procedure. Alternately the same samples can be sent out to a number of labs (Real and control samples). The results should be consistent. This procedure would identify labs that have consistently higher results for all samples. This process is called Round Robin testing
Why is destaining done after staining in agarose gel serum electrophoresis?
To remove extra Etbr and higher background fluorescence.
Why was it important for mendel study such a large sample of pea plants?
(Apex Learning) A higher sample size gives more accurate results.
Why would a scientist use a higher versus a lower percent agarose solution when preparing a gel to separate DNA?
Different percentages have different resolving powers. There is no one agarose percentage that is suitable for all sizes of DNA - you must chose the percentage best for resolving the sizes of DNA you are examining. If your agarose concentration is too dense for the size of your DNA fragments, the DNA will barely migrate through the gel. If the agarose concentration is too dilute for the size of your DNA, it will run straight through the gel without resolving into sharp bands. Generally speaking you use higher percentages if you want to resolve smaller DNA fragments and lower percentages if you want to resolve larger DNA fragments. Small DNA fragments need high percentages or else they'd run straight through the gel without being resolved into bands. Large DNA fragments need low percentages to permit them to migrate into the gel.
The higher the proof or alcohol percentage the faster the absorption?
the higher the proof or alcohol percentage the faster the absorption
The amount of 180 is what percentage higher then 135?
33.33% higher.
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A higher percentage of daughter isotopes present in a sample, the older the rock is.
Where is district wise kerala higher secondary results available for download?
Kerala Higher Secondary results can be downloaded from the below website. keralaresults.nic.in
Higher doses of barbiturates results in?
chepa
A higher albedo means there will be what?
A higher albedo means that a higher percentage of incoming light is reflected.
What is the relationship between the size of the DNA fragment and the distance that it migrates from the well?
. Because DNA is a negatively charged molecule, it will migrate through the gel toward the positive electrode (recall that opposite charges attract). The rate of migration of DNA through the agarose depends on the size of the DNA fragment. The smaller the fragment, the faster it can move through the gel. Another important factor is the concentration of agarose in the gel. The higher the concentration of agarose, the more it slows down the movement of all the DNA fragments.
