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Q: In a chromatography experiment chlorophyll pigments are separated using paper. What is the stationary phase in this experiment?
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Why is alcohol used when testing to see if chlorophyll is needed for photosynthesis?

Chlorophyll dissolves (becomes mobilized) in alcohol. Once mobilized, it can be separated from the other pigments by chromatography.


Iupac definition of chromatography?

"Chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of which is stationary while the other moves in a definite direction."


Is chromatography physically or chemically separated?

bakugan


A homogeneous mixture can be separated by what methods?

chromatography.


Why can't polymers be analysed by gas chromatography?

polymers are not volatile.. therefore, can't be separated by Gas Chromatography


What type of species can be separated by HPLC but not by gas liquid chromatography?

mixture of enantiomers can be separated by HPLC


What are disadvatages of chromatograph?

chromatography has many varieties -paper chromatography, sometime complexe mixtures cant be separated, TLC plates do not have long stationary phases -gaz chromatography: the molecule should be volatile -Chiral Chromatography can be expensive - Ion Exchange or Ion Chromatography: Turbidity should be low below 10ppm -Size Exclusion Chromatography: low resolution technique which gives few peaks and requires large differences in molecular weight for resolution -Gel chromatography: the target protein frequently becomes an abundant substrate for proteases that may also be present in the mixture. Another disadvantage is low sample handling.


The quantity of a substance separated by gas chromatography can be determined by its?

penis


What kind of species can be separated by high performance liquid chromatography but not gas chromatography?

HPLC can separate nonvolatile species and GC cannot.


Is qualitative determination using gas chromatography the best separation technique?

The quantity of a substance separated by gas chromatography can be determined by its?


Substances that may be separated into color components using chromatography?

gangnam lng :)


Separate the colors from black?

Colors from black can be separated by a process called Chromatography.