For European colonists, the lows came before 1585 (when the Roanoke Colony was established) and from 1590 to 1607 (Roanoke vanished, Jamestown was a success).
For the Native American population, it can only be conjectured, as no records were kept.
Harper's Ferry, WV (elev. 240 above sea level)
about 5
Vatican City770 people
the mountains and basinsrejon has the lowest population
The lowest estimate of the population is 50,000.
The most recent Census estimate (2007) was 1,812,035.
New Hampshire
Antarctica has the lowest population density of any continent. It has no permanent residents, and the population consists only of temporary researchers and scientists, resulting in a near-zero population density.
The population of Bayonne, France was 44,500 in the year 2007. Since 1968 the population of Bayonne has remained at a steady 42,000 people hitting its lowest in 1999 with 40,0113.
Alaska, because it has the lowest population of people in general.
Virginia was admitted into the Union on June 25, 1788 becoming the 10th state to join the union.
Europe is currently the third most populated continent with about 800,000,000 inhabitants. Europe's population growth rate has been decreasing due to recent trends and events, however, it is expected to increase though not soon. Anyways, Europe is not expected to reach the spot of the lowest populated continent, at least not in any time that is predictable.