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What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
What are staining artifacts?
Staining artifacts are unintended or undesirable effects in the sample caused by the staining process in microscopy. They can include uneven background staining, non-specific binding of the dye, precipitates forming during the staining process, or artifacts caused by improper fixation or tissue processing. Staining artifacts can affect the accuracy and interpretation of the results.
What are some good concrete staining designs?
To find good concrete staining you can visit lowes.com or homedepot.com.
Why is the technique called negative staining?
Because the cell wall repels the binding of the negative stain therefore the cells do not stain. Because of this the background is stain with the dye used and the bacteria remain colorless. Basically your staining the background, that is, you are not directly staining the cells.
Why is destaining done after staining in agarose gel serum electrophoresis?
To remove extra Etbr and higher background fluorescence.
How can a positive result occur during capsule staining?
Capsules may be revealed by methods such as Maneval's method. This method utilizes negative staining, where the background is stained revealing an unstained structure: the bacterial capsule.
Why EDTA is not dissolved in water?
EDTA is soluble in water.
What is the basicity of EDTA?
the basicity of EDTA is 4
What is EDTA whole blood?
EDTA whole blood is whole blood that has been drawn into a tube with EDTA in it. The EDTA is added to transport samples and prevents the blood from clotting.
Why an acidic stain is used in negative staining?
Acidic dyes are negatively-charged dyes. Since bacteria are also negatively-charged, they will repel the acidic dyes. So, instead of staining the bacterium itself, it will be the background that will be colorized....
Is sodium chromate the same as edta?
No. Edta is Ethylenediaminetetraacetic acid
