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Answered 2009-11-20 17:32:54

Hay infusion is a method of making a suitable home for your microorganisms to grow, this is achieved by boiling hay with water. This results to a broth, rich with carbohydrates and other "food" for your microorganisms extracted from the hay you used. Now, to make your microorganisms grow in numbers in that infusion, you need to get a little amount of the sample that you want to test for the presence of microorganisms. Example, getting a few milliliters of pond water, then pouring it to your hay-infusion. We will leave this for 24-48 hours so that the microorganisms will be able to multiply and grow in your infusion. Now, regarding the question on prokaryotic and eukaryotic cells... These two are actually the types of cells animals may have. Prokaryotic cells are said to be primitive and are commonly seen in bacteria, while eukaryotic cells are more advanced cells seen in higher forms of life like us. However, in the infusion you prepared, after pouring the sample that you want to be tested, we will see a variety of prokaryotic and eukaryotic microorganisms swimming about. Both types are present and it's a matter of identifying one from the other.

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A hay infusion is a culture made from water collected from a pond, lake, stream or puddle.

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