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Gram staining allows you to visualize bacteria and cells. I would only follow a Gram stain with a KOH test if I didn't find anything pathogenic or interesting to look at on the Gram stained slide.
The KOH test breaks down large structures (usually hairs) that may be holding or blocking the visualization of pathogenic organisms. It is very useful on skin scrapings to look for ringworm, but otherwise it's not that useful clinically.
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What is the staining process for separating bacteria?
This is known as a gram test.
What test would be useful to differentiate between the two genera if you see Gram positive cocci under the microscope but you cant quite tell the arrangement of the cells?
Gram staining would be the test that would be useful to differentiate between the two genera if you see gram positive cocci under the microscope, especially if you can't quite tell the arrangement. Another name for the gram staining test is Gram's method.
What is gram positive and gram negative?
Gram-positive is a result of the Gram staining technique, developed by Danish scientist Hans Christian Gram (1853 - 1938). Bacteria can have two types of cell walls. Gram-Positive bacteria have a relatively thick layer of peptidoglycan. Gram-negative bacteria have a much thinner layer of peptidoglycan. There are 4 basic steps to a Gram-staining. # The smear is flooded with a primary stain (such as crystal violet). This generally ends up staining all cells within the smear. # The smear is rinsed to remove excess dye, and a mordant such as an iodine solution is flooded onto the smear. A mordant is a substance that increases the affinity of cellular components for a dye. # The smear is rinsed again, removing all excess dye. The smear is then briefly washed with a 95% alcohol or a alcohol-acetone mixture. This mixture acts as a decolorizing agent. If the color is washed away then you are dealing with a Gram-negative bacteria (thin layer of peptidoglycan). # A counterstain is applied to the rest of the smear as a contrasting color to the now colorless Gram-negative bacteria (typically the red dye safranin). The Gram-positive bacteria remains violet because the dye was never decolorized because of the thick peptidoglycan cell wall. The gist of this is that Gram-positive bacteria will absorb the dye within their thick peptidoglycan cell wall component and resist the effects of decolorizing alcohol. Gram-negative bacteria will easily lose the dye from their thin peptidoglycan component of the cell wall. The significance of this test allows Microbiologists, Doctors, etc to fight the bacteria with certain specific antibiotics.
What is an easy way to remember that gram negative bacteria turn red?
One way to remember that gram negative bacteria turn red is to think of the phrase "red is negative." This reminds you that when performing a Gram staining test, gram negative bacteria will take on the red color.
What does a student need to know about a microbe in order to start researching in bergey's manual of systematic bacteriology?
A student would need to do as many test as possible to have a list of what it could be for the manual. Bergey's manual of systematic bacteriology helps student identify bacteria after test has been done for gram staining, acid fast test etc.
What is the staining process for separating bacteria?
This is known as a gram test.
Is Gram staining considered a moderate or high complexity test by CLIA standards?
high
What test would be useful to differentiate between the two genera if you see Gram positive cocci under the microscope but you cant quite tell the arrangement of the cells?
Gram staining would be the test that would be useful to differentiate between the two genera if you see gram positive cocci under the microscope, especially if you can't quite tell the arrangement. Another name for the gram staining test is Gram's method.
What are the gram positive diplococci?
They are bacteria, their morphology is basically two cocci cells fused with one another. They test positive for the Gram staining test. An example of GPDC is the bacteria genus Streptococcus pneumoniae, the major causative agent for pneumonia; a pathological lung condition
What is the purpose of control test in gram stain?
Gram staining is a simple staining test that simply identifies the two main groups of bacteria. Gram positive, and gram negative. Down a microscope, gram pos look like a dark blue/purple colour, and gram neg look red. It is to do with what the wall of the bacteria comprises of, and without going into too much detail, certain drugs work on gram pos bacteria, and others wont. Likewise for gram neg.
What staining test causes the corneal abrasions to appear bright green?
fluorescein staining
Would it be useful to perform a gram stain on a mixed culture?
The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.
Why must fresh bacterial cultures be used in a Gram stain?
Why must young cultures be used when doing a Gram stain Young cultures must be used so the crystal violet can stick to the cell walls of Gram positive bacteria. The cell walls break down in old cultures and the staining process is not accurate
What is gram positive and gram negative?
Gram-positive is a result of the Gram staining technique, developed by Danish scientist Hans Christian Gram (1853 - 1938). Bacteria can have two types of cell walls. Gram-Positive bacteria have a relatively thick layer of peptidoglycan. Gram-negative bacteria have a much thinner layer of peptidoglycan. There are 4 basic steps to a Gram-staining. # The smear is flooded with a primary stain (such as crystal violet). This generally ends up staining all cells within the smear. # The smear is rinsed to remove excess dye, and a mordant such as an iodine solution is flooded onto the smear. A mordant is a substance that increases the affinity of cellular components for a dye. # The smear is rinsed again, removing all excess dye. The smear is then briefly washed with a 95% alcohol or a alcohol-acetone mixture. This mixture acts as a decolorizing agent. If the color is washed away then you are dealing with a Gram-negative bacteria (thin layer of peptidoglycan). # A counterstain is applied to the rest of the smear as a contrasting color to the now colorless Gram-negative bacteria (typically the red dye safranin). The Gram-positive bacteria remains violet because the dye was never decolorized because of the thick peptidoglycan cell wall. The gist of this is that Gram-positive bacteria will absorb the dye within their thick peptidoglycan cell wall component and resist the effects of decolorizing alcohol. Gram-negative bacteria will easily lose the dye from their thin peptidoglycan component of the cell wall. The significance of this test allows Microbiologists, Doctors, etc to fight the bacteria with certain specific antibiotics.
What is an easy way to remember that gram negative bacteria turn red?
One way to remember that gram negative bacteria turn red is to think of the phrase "red is negative." This reminds you that when performing a Gram staining test, gram negative bacteria will take on the red color.
What does a student need to know about a microbe in order to start researching in bergey's manual of systematic bacteriology?
A student would need to do as many test as possible to have a list of what it could be for the manual. Bergey's manual of systematic bacteriology helps student identify bacteria after test has been done for gram staining, acid fast test etc.
What test is done to diagnose a scratched cornea of the eye?
Fluorescien staining
