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Different methods of protein estimation?
This question has to be answered depending upon the meaning of a research project that involves proteins. The estimation of proteins can be done to know the protein fraction of a sample collected from the field, either if the protein content will be isolated to be studied, or to remove protein fraction from the sample (sometimes the investigator does not want that proteolytic enzymes chew a target molecules or cellular structures). Another application is when an enzymatic reaction is going to be performed to digest a particular protein in an aliquot, the protein content is crucial for stoichometric purposes. On the other hand, if the research imply the work with nucleic acids, is very important "to inactivate" the nucleolytic enzymes present in cytoplasmic fluids from lysed cells with specific inactivators, chemical or biochemical, added in correct levels according to protein contents.
Why protein estimation is done at 660 nanometer in folin lowry method?
Protein estimation in the Folin-Lowry method is done at 660 nanometers because this wavelength corresponds to the highest absorption peak of the complex formed between proteins and the Folin-Lowry reagent. This wavelength is ideal for accurately measuring the concentration of proteins present in a sample based on the colorimetric reaction that occurs.
What is the standard reference method for protein determination?
The standard reference method for protein determination is the Kjeldahl method. This method involves digesting the protein sample with concentrated sulfuric acid and then measuring the amount of nitrogen present, which is used to calculate the protein content.
How is the Gerber method of fat estimation done?
The Gerber method of fat estimation involves mixing milk with sulfuric acid and centrifuging it to separate the fat. The fat layer is then measured and compared to a standard to determine the fat content of the milk. It is a widely used method in dairy industry for accurate estimation of fat content in milk.
How can one test for protein in a sample?
One can test for protein in a sample by using a method called the Biuret test. This test involves adding a reagent to the sample, which causes a color change if protein is present. The intensity of the color change can indicate the amount of protein in the sample.
What is an invasive method of protein estimation?
gand mara
How long the blue colour is stable in protein estimation by lowry's method?
60 minutes 60 minutes
Is lowry's method an invasive method?
No. It is a non-invasive method
What method are used for the estimation of molecular weight of protein?
ofcoz the protein migrate so long as it is charged ,once it become neutral it will stop migrating
Different methods of protein estimation?
This question has to be answered depending upon the meaning of a research project that involves proteins. The estimation of proteins can be done to know the protein fraction of a sample collected from the field, either if the protein content will be isolated to be studied, or to remove protein fraction from the sample (sometimes the investigator does not want that proteolytic enzymes chew a target molecules or cellular structures). Another application is when an enzymatic reaction is going to be performed to digest a particular protein in an aliquot, the protein content is crucial for stoichometric purposes. On the other hand, if the research imply the work with nucleic acids, is very important "to inactivate" the nucleolytic enzymes present in cytoplasmic fluids from lysed cells with specific inactivators, chemical or biochemical, added in correct levels according to protein contents.
What is front end estimation?
Front end estimation- An estimation method in which the front digits are added or subtracted
What is protein estimation?
here the amount of protein in a sample can be determined, using different protocols, wherein the reagent mixture( ex: FC reagent) which when added to the sample containing protein reacts with the specific amino acids giving colour, thus the amount of protein in a sample can be estimated and the data used for further protein studies.
What is a method for math in a estimation?
round or rounding
Why BSA is used as standard for protein estimation?
The Bradford assay (BSA) is commonly used for protein estimation because it is quick, sensitive, and compatible with a wide range of proteins. BSA is a reliable method for measuring protein concentration due to its colorimetric detection of the dye-protein complex, making it a popular choice in biochemical research and diagnostics.
Why protein estimation is done at 660 nanometer in folin lowry method?
Protein estimation in the Folin-Lowry method is done at 660 nanometers because this wavelength corresponds to the highest absorption peak of the complex formed between proteins and the Folin-Lowry reagent. This wavelength is ideal for accurately measuring the concentration of proteins present in a sample based on the colorimetric reaction that occurs.
Can one cost estimation method can be used in combination with another method?
Yes. True
Can one cost estimation method be used in combination with another method?
Yes. True
