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Are endospores acid fast?
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
Are acid fast bacteria gram positive or negative?
it is positive
Is klebsiella pneumoniae acid fast?
gram positive
Is staphylococcus aureus acid fast?
No. in comparison to M. smegmatis which is acid fast positive. e. coli will appear blue when tested with the Zeihl-Neelsen acid fast stain
Is the mycobacterium smegmatis positive to acid-fast?
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
What gram reaction do you expect from acid-fast bacteria?
Gram positive
Which group of microorganism is liable to stain positive with an acid fast stain and why?
Gram positive, cos they have thick cell wall
Is Micrococcus luteus acid fast negative?
Micrococcus leteus is negative to VP test.
Is mycobacterium phlei gram positive or negative?
Although mycoplasma lack a cell wall and therefore test gram negative, they are considered to be descendents of 'nonsporulating and endospore forming gram-positive bacteria' (Madigan et al., 2009), such as Lactobacilli, Bacilli and Streptococci, (Dandekar et al., 2002), which have lost their cell wall. Mycoplasmas are therfore classed as gram- positive bacteria. This is supported by ribosomal RNA and DNA analysis.
Is a Gram stain an adequate substitute for an acid fast stain?
So few organisms are acid-fast, the acid fast stain is used only when infection by an acid-fast organisms is suspected.
Does an isolated non-acid fast gram-positive catalase positive rods from a throat represent a disease state?
yes bc its only normal on skin not in your throat
Cite the purpose of following reagents in a differential staining procedure?
To distinguish between acid fast positive and acid fast negative bacteria. Acid fast positive bacteria will stain red to pink color and acid fast negative bacteria will stain blue. Acid fast positive bacteria have mycolic acid in their cell wall, which will stain with carbol fuchsin and not decolorize with acid alcohol. Acid fast negative bacteria do not have mycolic acid in their cell wall, and become decolorize with the acid alcohol. Counterstain of methylene blue needs to be done in order to see the acid fast negative.
