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Is the acid fast stain result positive or negative for the sample?
The acid-fast stain result is positive for the sample.
Is the acid fast staining result positive or negative for the sample?
The acid-fast staining result for the sample is positive.
Does yogurt stain gram positive or gram negative?
Yogurt contains mostly lactic acid bacteria, which are Gram-positive bacteria. This means they will stain purple under a Gram stain.
Is Micrococcus luteus acid fast negative?
Yes, Micrococcus luteus is typically Gram-positive and non-acid-fast, meaning it does not retain the carbol fuchsin stain when subjected to acid-alcohol treatment in acid-fast staining methods like the Ziehl-Neelsen stain or Kinyoun stain.
Do acid-fast bacteria stain gram-negative when subjected to the gram stain?
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Is the acid fast stain result positive or negative for the sample?
The acid-fast stain result is positive for the sample.
Is the acid fast staining result positive or negative for the sample?
The acid-fast staining result for the sample is positive.
Does yogurt stain gram positive or gram negative?
Yogurt contains mostly lactic acid bacteria, which are Gram-positive bacteria. This means they will stain purple under a Gram stain.
Is Micrococcus luteus acid fast negative?
Yes, Micrococcus luteus is typically Gram-positive and non-acid-fast, meaning it does not retain the carbol fuchsin stain when subjected to acid-alcohol treatment in acid-fast staining methods like the Ziehl-Neelsen stain or Kinyoun stain.
Do acid-fast bacteria stain gram-negative when subjected to the gram stain?
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Cite the purpose of following reagents in a differential staining procedure?
To distinguish between acid fast positive and acid fast negative bacteria. Acid fast positive bacteria will stain red to pink color and acid fast negative bacteria will stain blue. Acid fast positive bacteria have mycolic acid in their cell wall, which will stain with carbol fuchsin and not decolorize with acid alcohol. Acid fast negative bacteria do not have mycolic acid in their cell wall, and become decolorize with the acid alcohol. Counterstain of methylene blue needs to be done in order to see the acid fast negative.
What are the differences between the Gram stain and acid-fast stain techniques in microbiology?
The Gram stain technique is used to differentiate bacteria into two groups based on their cell wall composition (Gram-positive and Gram-negative), while the acid-fast stain technique is used to detect bacteria that have a waxy cell wall, such as Mycobacterium species.
Is maneval's stain an acid stain?
Yes, Maneval's stain is an acid-fast stain used in microbiology to detect acid-fast bacteria such as Mycobacterium species. It involves using acid-alcohol to decolorize non-acid-fast bacteria while acid-fast bacteria retain the stain due to their waxy cell wall.
Is the mycobacterium smegmatis positive to acid-fast?
Yes, Mycobacterium smegmatis is positive for acid-fast staining due to its thick, waxy cell wall. This characteristic allows it to resist decolorization by acid-alcohol during the staining process, leading to retention of the primary stain (carbol fuchsin) and appearing pink or red under a microscope.
Does carbolfuchsin stain acid fast negative cells?
Yes, carbolfuchsin can stain acid-fast negative cells. This red dye can easily get into their thin cell wall lipids due to its solubility.
How does acid-fast bacteria differ from gram bacteria?
Acid-fast and Gram stain are two different types of staining technique. Bacteria that stain with acid-fast techniques are called "acid fast bacteria." Bacteria that don't stain with acid-fast techniques are called "not acid fast." At the same time, bacteria can be "Gram-positive" or "Gram-negative." For example, Nocardia is a bacterium that is acid-fast and Gram-positive. Usually the reason people care about these designations is in order to figure out what species a bacteria is. The issue usually arises in the context of a patient's sample that has grown bacteria. At first the doctors won't be sure which bacteria it is. They'll have guesses based on how the patient presented, but they won't know, and they want to know because that will help them pick the best treatment. A first step toward "speciating" the bacteria is to do a Gram stain on it. This is because for historical reasons determining whether a bacteria is Gram-positive or Gram-negative goes a long way toward speciating it. Acid-fast staining is less common, and is used mostly for diagnosing tuberculosis (caused by Mycobacteria, which are acid-fast bacteria). There are details about the cell walls that determine whether an organism will stain with Gram stain (thick walls without mycolic acids) or acid-fast, but hopefully this answers your question.
Is acid fast bacteria gram positive or negative?
Acid-fast bacteria are gram-positive.
