0
What else can I help you with?
Can use carbolfuchsin as a counterstain?
Carbolfuchsin can be used as a counterstain in certain staining techniques, particularly in the acid-fast staining method used to detect acid-fast bacteria like Mycobacterium tuberculosis. It helps to differentiate acid-fast bacteria, which retain the primary stain (carbolfuchsin), from non-acid-fast bacteria which are counterstained with a contrasting color.
What is carbolfuchsin?
This compound is basic.
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
The counter stain used in the Gram stain procedure is typically safranin or basic fuchsin, which stains Gram-negative bacteria pink or red. In the acid-fast stain procedure, the counter stain used is typically methylene blue or brilliant green, which stains non-acid-fast bacteria blue or green, allowing acid-fast bacteria to retain the primary stain color (carbolfuchsin).
What color is Brilliant green K in Acid fast cell?
Brilliant green K typically appears as a green color when used as a counterstain in Acid-fast staining procedures. It helps to differentiate non-acid-fast bacteria from Acid-fast cells like Mycobacterium spp, which retain the primary stain (carbolfuchsin) and appear red.
What is an acid-fast pathogen?
An acid-fast pathogen is a bacteria that is harmful to humans. They have cell walls that contain mycolic acid which is a lipid. Common Gram type staining techniques wont work with these cells. A special stain carbolfuchsin is used to penetrate the wall.After staining you wash with acid alcohol if the stain remains it is acid fast if it washes out it is non-acid fast.Mycobacterium tuberculosis is a well known acid-fast pathogen
Do acid-fast bacteria stain gram-negative when subjected to the gram stain?
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the PH would be what?
lol, we must be in the same class, b/c i have all of the same questions. did u figure out the answer? I think its a base ... qc the microbiology lab Cute, from the best that I could find some sources say pH 7 and others had given a pH 8. But could not find a solid answer. As a simple stain it would have to have a pH of <7. As we know, the charge on the cell is always going to be negative (an anion). In order to achieve a simple stain we want the stain attracted to the cell wall; therefore, the stain must be positively charged (a cation) for the opposite forces to attract. The more acidic the compound - the smaller its pH number with 7 being neutral. As we know, the [H]+ increases as the pH decreases becoming evermore positively charged. Simple answer: <7
Is the acid fast stain positive or negative in the sample?
The acid-fast stain is positive in the sample.
What color are non acid fast organisms after staining?
Acid-fast organisms are characterized by wax-like cell walls.Because the cell wall is so resistant to most compounds, acid-fast organisms require a special staining technique.1. The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. (violet or purple).2. This is further assisted by the addition of heat.3. The smear is then rinsed with a very strong decolorizer, which strips the stain from all non-acid-fast cells but does not permeate the cell wall of acid-fast organisms.4. The decolorized non-acid-fast cells then take up the counterstain. (safranin- red).Gram positive bacteria will stain purple.Gram negative bacteria will stain red/pink.
What makes a microorganism non-acid fast?
The low lipid content in the cells ----------------------------------------------- The above answer is not wrong. I'm just giving adding more information to it. Acid-fast staining is due to the high lipid content (mycolic acid) in the cell wall. Cells that do not have these mycolic acids do not absorb the carbolfuchsin. Microorganisms that have taken up the carbolfuchsin are not easily decolorized by the acid-alcohol step in the preparation procedure.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Is the acid fast stain result positive or negative for the sample?
The acid-fast stain result is positive for the sample.
