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What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Wiki User
∙ 16y ago
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Wiki User
∙ 16y ago
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What ia the Streptomyces sp gram stain and shape?
Gram Positive Rods (Filamentous is the exact term for the type of rod)
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
What happens when you leave a stain on the bacterial smear for too long?
If the bacterial cell are under stained then they will loose the stain of dye when wash with alocohol or may be simply by water which then cause a problem in identifying the cell type as e.g. in case of gram staining if the cell do not stain properly with methylene blue then they will loose the stain when washed and will counter stain with saffranin so the gram +ve will show the gram -ve colour.
WHAT ARE the different reagents in gram stain method and its uses?
Mordant is the reagent used in the gram stain method. By using this type of reagent bacteria are more easily seen. It is important to a doctor to know what kind of bacteria is present so that he knows what to prescribe to a patient to get rid of the bacteria.
What is Beta-lactamase positive?
Beta-lactamases are enzymes produced by bacteria. Gram-positive bacteria can produce beta-lactamases. This type of bacteria takes up the crystal violet stain in gram staining.
Can you tell an organisms gram stain by simple staining?
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
What is gram staining used to distinguish?
The first stain is crystal violet, next iodine is added which acts as a mordent. Then, alcohol is added which will wash away the purple color from any gram negative bacteria. The purple color will stay in the gram positive bacteria due to their thich peptidoglycan layer. Lastly, Safranin is added as a counter stain. Gram - is red Gram + is purple
What ia the Streptomyces sp gram stain and shape?
Gram Positive Rods (Filamentous is the exact term for the type of rod)
What type of organism would you look for in a Gram stain of the blood or cerebrospinal fluid in a menin gococcal case?
A gram-negative diplococcus
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
What happens when you leave a stain on the bacterial smear for too long?
If the bacterial cell are under stained then they will loose the stain of dye when wash with alocohol or may be simply by water which then cause a problem in identifying the cell type as e.g. in case of gram staining if the cell do not stain properly with methylene blue then they will loose the stain when washed and will counter stain with saffranin so the gram +ve will show the gram -ve colour.
Is there a gram negative streptococcus?
Yes, gram negative streptococcus does exists. This is a type of bacteria that cannot retain a violet stain after the decolonization step of testing.
What is th epurpose of a gram stain?
To ID the type of bacteria found in a cut or would. Knowing this will determine the type of antibiotic to use.
Why would a gram stain be ordered during surgery?
to determine a type of bacteria. This question is very vague. I will assume they are doing surgery on something that is infected and to help identify the the bacteria causing the infection, gram stain would be ordered.
What is the basic for gram stain results between different bacteria?
its the difference in cell wall of both the type of bacteria
Is the disease Lyme disease gram stain positive?
Spirochetes are not considered Gram positive or Gram negative. They tend to stain pink due to the last safrinin dye in the staining process, but its cell wall structure should make it Gram negative.
Is HIV gram positive or gram negative?
HIV is a virus and, therefore, is neither Gram positive nor Gram negative. The Gram stain is used to identify bacteria as either Gram negative or positive, depending upon the type of cell wall the bacteria has. The Gram stain cannot be used on viruses.
